Tested Applications
| Positive WB detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26230-1-AP targets OBFC1 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag23868 Product name: Recombinant human OBFC1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 29-368 aa of BC017400 Sequence: LYIRDILDMKESRQVPGVFLYNGHPIKQVDVLGTVIGVRERDAFYSYGVDDSTGVINCICWKKLNTESVSAAPSAARELSLTSQLKKLQETIEQKTKIEIGDTIRVRGSIRTYREEREIHATAYYKVDDPVWNIQIARMLELPTIYRKVYDQPFHSSALEKEEALSNPGALDLPSLTSLLSEKAKEFLMENRVQSFYQQELEMVESLLSLANQPVIHSACSDQVNFKKDTTSKAIHSIFKNAIQLLQEKGLVFQKDDGFDNLYYVTREDKDLHRKIHRIIQQDCQKPNHMEKGCHFLHILACARLSIRPGLSEAVLQQVLELLEDQSDIVSTMEHYYTAF Predict reactive species |
| Full Name | oligonucleotide/oligosaccharide-binding fold containing 1 |
| Observed Molecular Weight | 42 kDa |
| GenBank Accession Number | BC017400 |
| Gene Symbol | OBFC1 |
| Gene ID (NCBI) | 79991 |
| RRID | AB_2880435 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H668 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for OBFC1 antibody 26230-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
One reviewer (Daniel U., October 2024) rated this antibody 3/5 stars for Western Blot at a 1:2000 dilution in eHAP and RPE1 cells. They confirmed it detects a specific STN1 band validated by knockdown/knockout, but noted the antibody is very dirty, making it difficult to clearly see the actual STN1 band among background signals.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Daniel (Verified Customer) (10-24-2024) | The antibody detects a specific band for STN1 (validated by Knockdown/knockout). However, the antibody is very dirty, which can make it difficult to see the actual STN1 band.
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