OAS3 Polyclonal antibody

• Featured Product
• KD/KO Validated

OAS3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 21915-1-AP

Synonyms

p100, P/OKcl.4, EC:2.7.7.84, 2'-5'-oligoadenylate synthase 3, 2-5A synthase 3

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Validation Data Gallery

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  WB analysis using 21915-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21915-1-AP (OAS3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 21915-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 21915-1-AP (OAS3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 21915-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 21915-1-AP (OAS3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin cancer using 21915-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 21915-1-AP (OAS3 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 21915-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21915-1-AP (OAS3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 21915-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21915-1-AP (OAS3 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 21915-1-AP

  Immunofluorescent analysis of HeLa cells using 21915-1-AP (OAS3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A375 cells, human placenta tissue, HeLa cells
• Positive IHC detected in: human skin cancer tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IHC: See 2 publications below

Product Information

21915-1-AP targets OAS3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OAS3 fusion protein Ag16150
• Full Name: 2'-5'-oligoadenylate synthetase 3, 100kDa
• Calculated Molecular Weight: 1087 aa, 121 kDa
• Observed Molecular Weight: 100-120 kDa
• GenBank Accession Number: BC113746
• Gene Symbol: OAS3
• Gene ID (NCBI): 4940
• RRID: AB_2876880
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The 2-prime,5-prime oligoadenylate synthetases (OASs), such as OAS3, are interferon-induced proteins characterized by their capacity to catalyze the synthesis of 2-prime,5-prime oligomers of adenosine (2-5As). It is also named as p100 OAS and belongs to the 2-5A synthase family. OAS3 is involved in inhibition of cellular protein synthesis and in viral infection resistance. This antibody may have weak cross-reaction with OAS1 protein due to the highly homology.

Publications

Species	Application	Title
human	WB	Science; A prenylated dsRNA sensor protects against severe COVID-19.; Authors - Arthur Wickenhagen ; KO Validated
human	WB	Nat Cell Biol; Redox regulation of m6A methyltransferase METTL3 in β-cells controls the innate immune response in type 1 diabetes; Authors - Dario F De Jesus
mouse	WB	Mol Cell; RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals composition dynamics in embryonic tissue, cancer cells, and macrophages; Authors - Teodorus Theo Susanto
human		Nat Commun; Large-scale and high-resolution mass spectrometry-based proteomics profiling defines molecular subtypes of esophageal cancer for therapeutic targeting.; Authors - Wei Liu
human	WB	J Invest Dermatol; OAS1, OAS2, and OAS3 Contribute to Epidermal Keratinocyte Proliferation by Regulating Cell Cycle and Augment Type I Interferon-Induced JAK1-STAT1 Phosphorylation in Psoriasis.; Authors - Yan-Zhou Huang ; KD Validated
human	WB	Virol Sin; EV71 3C protease cleaves host anti-viral factor OAS3 and enhances virus replication.; Authors - Xiaolei Zhou ; KD Validated