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OAS2 Polyclonal antibody

OAS2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF

Conjugate

Unconjugated

Cat no : 19279-1-AP

Synonyms

(2 5)oligo(A) synthase 2, 2 5 oligoadenylate synthase 2, 2 5A synthase 2, OAS2, p69 OAS / p71 OAS, p69OAS / p71OAS



Tested Applications

Positive WB detected inJurkat cells, human liver tissue, HL-60 cells, mouse lung tissue
Positive IP detected inJurkat cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 19279-1-AP is OAS2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OAS2 fusion protein Ag6170
Full Name 2'-5'-oligoadenylate synthetase 2, 69/71kDa
Calculated molecular weight 82 kDa
Observed molecular weight 66-71 kDa
GenBank accession numberBC049215
Gene symbol OAS2
Gene ID (NCBI) 4939
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The 2-prime,5-prime oligoadenylate synthetases (OASs), such as OAS2, are IFN-induced proteins characterized by their capacity to catalyze the synthesis of 2-prime,5-prime oligomers of adenosine (2-5As). OAS2 is also named as p69 OAS / p71 OAS. It has 3 isoforms(71/69/20 kDa) produced by alternative splicing. The OAS2 isozymes are 69-71 kDa and form dimers(PMID:17765707). Glycosylation is essential for its activity(PMID:9880569). Constitutive expression of the 69-kDa form of the OAS2 protein in human cells inhibited the replication of encephalomyocarditis virus (EMCV) but not that of VSV, Sendai virus, or reovirus(PMID:16501108). This antibody is specific to isoform p71 and isoform p69 of OAS2.

Protocols

Product Specific Protocols
WB protocol for OAS2 antibody 19279-1-APDownload protocol
IHC protocol for OAS2 antibody 19279-1-APDownload protocol
IF protocol for OAS2 antibody 19279-1-APDownload protocol
IP protocol for OAS2 antibody 19279-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochem Cell Biol

Impact of double-stranded RNA characteristics on the activation of human 2'-5'-oligoadenylate synthetase 2 (OAS2).

Authors - Amit Koul
humanIF

Sci Adv

IL-27 signaling activates skin cells to induce innate antiviral proteins and protects against Zika virus infection.

Authors - Jeffery T Kwock
humanWB

Nat Commun

Decoding non-canonical mRNA decay by the endoplasmic-reticulum stress sensor IRE1α

Authors - Adrien Le Thomas
humanIHC

Exp Dermatol

Spleen tyrosine kinase (SYK) is a potential target for the treatment of cutaneous lupus erythematosus patients.

Authors - Christine Braegelmann
humanWB

PLoS One

Spleen Tyrosine Kinase (Syk) Regulates Systemic Lupus Erythematosus (SLE) T Cell Signaling.

Authors - Grammatikos Alexandros P AP
  • KD Validated
humanWB

Mediators Inflamm

Expression of Interferon Effector Gene SART1 Correlates with Interferon Treatment Response against Hepatitis B Infection.

Authors - Yong Li