OAS2 Polyclonal antibody

• Featured Product
• KD/KO Validated

OAS2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

22

Cat no : 19279-1-AP

Synonyms

EC:2.7.7.84, 2'-5'-oligoadenylate synthase 2, 2-5A synthase 2, (2-5')oligo(A) synthase 2

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Validation Data Gallery

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  WB analysis using 19279-1-AP

  IFN alpha treated A549 cells were subjected to SDS PAGE followed by western blot with 19279-1-AP (OAS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 19279-1-AP

  IP result of anti-OAS2 (IP:19279-1-AP, 4ug; Detection:19279-1-AP 1:500) with Jurkat cells lysate 2280 ug.

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  IHC staining of human colon cancer using 19279-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 19279-1-AP (OAS2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 19279-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19279-1-AP (OAS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 19279-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19279-1-AP (OAS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 19279-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 19279-1-AP (OAS2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 19279-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19279-1-AP (OAS2 Antibody) at dilution of 1:50.

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  IHC staining of human breast cancer using 19279-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19279-1-AP (OAS2 Antibody) at dilution of 1:50.

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  IF Staining of HepG2 using 19279-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using OAS2 antibody (19279-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: IFN alpha treated A549 cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human colon cancer, human breast cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 14 publications below
• IHC: See 4 publications below
• IF: See 4 publications below

Product Information

19279-1-AP targets OAS2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OAS2 fusion protein Ag6170
• Full Name: 2'-5'-oligoadenylate synthetase 2, 69/71kDa
• Calculated Molecular Weight: 82 kDa
• Observed Molecular Weight: 66-71 kDa
• GenBank Accession Number: BC049215
• Gene Symbol: OAS2
• Gene ID (NCBI): 4939
• RRID: AB_10642832
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The 2-prime,5-prime oligoadenylate synthetases (OASs), such as OAS2, are IFN-induced proteins characterized by their capacity to catalyze the synthesis of 2-prime,5-prime oligomers of adenosine (2-5As). OAS2 is also named as p69 OAS / p71 OAS. It has 3 isoforms(71/69/20 kDa) produced by alternative splicing. The OAS2 isozymes are 69-71 kDa and form dimers(PMID:17765707). Glycosylation is essential for its activity(PMID:9880569). Constitutive expression of the 69-kDa form of the OAS2 protein in human cells inhibited the replication of encephalomyocarditis virus (EMCV) but not that of VSV, Sendai virus, or reovirus(PMID:16501108). This antibody is specific to isoform p71 and isoform p69 of OAS2.

Publications

Species	Application	Title
human	WB	Science; A prenylated dsRNA sensor protects against severe COVID-19.; Authors - Arthur Wickenhagen
human	WB	Nat Cell Biol; Redox regulation of m6A methyltransferase METTL3 in β-cells controls the innate immune response in type 1 diabetes; Authors - Dario F De Jesus
human	WB	Nat Commun; Decoding non-canonical mRNA decay by the endoplasmic-reticulum stress sensor IRE1α; Authors - Adrien Le Thomas
human	IF	Sci Adv; IL-27 signaling activates skin cells to induce innate antiviral proteins and protects against Zika virus infection.; Authors - Jeffery T Kwock
human	WB	Sci Immunol; OAS1/RNase L executes RIG-I ligand-dependent tumor cell apoptosis.; Authors - Daniel F R Boehmer
human,mouse	IF	J Invest Dermatol; Skin Injury Activates A Rapid TRPV1-dependent Antiviral Protein Response.; Authors - Vivian Lei