Validation Data Gallery
|Positive WB detected in||IFN alpha treated A549 cells|
|Positive IP detected in||Jurkat cells|
|Positive IHC detected in||human liver cancer tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
19279-1-AP targets OAS2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||OAS2 fusion protein Ag6170|
|Full Name||2'-5'-oligoadenylate synthetase 2, 69/71kDa|
|Calculated molecular weight||82 kDa|
|Observed molecular weight||66-71 kDa|
|GenBank accession number||BC049215|
|Gene ID (NCBI)||4939|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
The 2-prime,5-prime oligoadenylate synthetases (OASs), such as OAS2, are IFN-induced proteins characterized by their capacity to catalyze the synthesis of 2-prime,5-prime oligomers of adenosine (2-5As). OAS2 is also named as p69 OAS / p71 OAS. It has 3 isoforms(71/69/20 kDa) produced by alternative splicing. The OAS2 isozymes are 69-71 kDa and form dimers(PMID:17765707). Glycosylation is essential for its activity(PMID:9880569). Constitutive expression of the 69-kDa form of the OAS2 protein in human cells inhibited the replication of encephalomyocarditis virus (EMCV) but not that of VSV, Sendai virus, or reovirus(PMID:16501108). This antibody is specific to isoform p71 and isoform p69 of OAS2.
|Product Specific Protocols|
|WB protocol for OAS2 antibody 19279-1-AP||Download protocol|
|IHC protocol for OAS2 antibody 19279-1-AP||Download protocol|
|IF protocol for OAS2 antibody 19279-1-AP||Download protocol|
|IP protocol for OAS2 antibody 19279-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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OAS1/RNase L executes RIG-I ligand-dependent tumor cell apoptosis.
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