OAS1/3 Polyclonal antibody

• Featured Product
• KD/KO Validated

OAS1/3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Publications

28

Reviews (1)

Cat no : 14955-1-AP

Synonyms

OAS1, EC:2.7.7.84, E18/E16, 2'-5'-oligoadenylate synthase 1, 2-5A synthase 1

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Validation Data Gallery

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  WB analysis using 14955-1-AP

  Untreated and IFN alpha treated HeLa cells were subjected to SDS PAGE followed by western blot with 14955-1-AP (OAS1/3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14955-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14955-1-AP (OAS1/3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 14955-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14955-1-AP (OAS1/3 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 14955-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14955-1-AP (OAS1/3 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver using 14955-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 14955-1-AP (OAS1/3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 14955-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 14955-1-AP (OAS1/3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of A431 using 14955-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using OAS1/3 antibody (14955-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: IFN alpha treated HeLa cells, mouse brain tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 25 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Product Information

14955-1-AP targets OAS1/3 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OAS1/3 fusion protein Ag6793
• Full Name: 2',5'-oligoadenylate synthetase 1, 40/46kDa
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: ~40 kDa, 100-120 kDa
• GenBank Accession Number: BC000562
• Gene Symbol: OAS1
• Gene ID (NCBI): 4938
• RRID: AB_2158292
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The 2-prime,5-prime oligoadenylate synthetases (OASs), such as OAS1, are interferon-induced proteins characterized by their capacity to catalyze the synthesis of 2-prime,5-prime oligomers of adenosine (2-5As). OAS1 (type I enzymes) has some isoforms with the MW of 40, 42, 44, 46, and 48 kDa (PMID:12590567, 19383565). OAS1 is a strong candidate for determining susceptibility or resistance to viral infections(PMID:15732009). This antibody may have cross-reaction with OAS3 protein due to the high homology.

Publications

Species	Application	Title
human	WB	Science; A prenylated dsRNA sensor protects against severe COVID-19.; Authors - Arthur Wickenhagen ; KO Validated
human	WB	Nat Cell Biol; Redox regulation of m6A methyltransferase METTL3 in β-cells controls the innate immune response in type 1 diabetes; Authors - Dario F De Jesus
human		Adv Sci (Weinh); The CRISPR-Cas13a Gene-Editing System Induces Collateral Cleavage of RNA in Glioma Cells.; Authors - Qixue Wang
human	WB	PLoS Biol; Resurrection of 2'-5'-oligoadenylate synthetase 1 (OAS1) from the ancestor of modern horseshoe bats blocks SARS-CoV-2 replication; Authors - Spyros Lytras
mouse	WB	Autophagy; USP19 (ubiquitin specific peptidase 19) promotes TBK1 (TANK-binding kinase 1) degradation via chaperone-mediated autophagy.; Authors - Xibao Zhao
human	WB	J Invest Dermatol; OAS1, OAS2, and OAS3 Contribute to Epidermal Keratinocyte Proliferation by Regulating Cell Cycle and Augment Type I Interferon-Induced JAK1-STAT1 Phosphorylation in Psoriasis.; Authors - Yan-Zhou Huang ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Seong (Verified Customer) (11-27-2018)

OAS1 polyclonal antibody (14955-1-AP) did work by Western Blot (1:1,500) of ARPE-19 cells induced with interferon gamma but the band was very weak in uninduced cells.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1,500
• Cell Tissue Type: human ARPE-19 cells