NeutraKine® TNF-alpha Monoclonal antibody, PBS Only

NeutraKine® TNF-alpha Monoclonal Antibody for Neutralization, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

Neutralization, ELISA

Conjugate

Unconjugated

CloneNo.

11H8A5

Cat no : 69002-1-PBS

Synonyms

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Validation Data Gallery

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  Neutralization experiment of NeutraKine® TNF-alpha using 69002-1-Ig (same clone as 69002-1-PBS)

  Recombinant human TNF alpha (Cat.NO. HZ-1014) inhibits the growth of L-929 cell line in the presence of actinomycin D in a dose dependent manner (blue curve, refer to bottom X-left Y). The activity elicited by recombinant human TNF alpha (0.05 ng/mL HZ-1014) is neutralized by mouse anti-human TNF alpha monoclonal antibody 69002-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 10-50 ng/mL. The NeutraControl mouse anti-human TNF alpha monoclonal antibody 69502-1-Ig, generated from the same immunogen, could recognize human TNF alpha, but could not neutralize human TNF alpha at the range of 0.128-10000ng/mL (green curve, refer to top X-right Y). This data was developed using the same antibody clone with 69002-1-PBS in a different storage buffer formulation.

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  ELISA experiment of Recombinant protein using 69002-1-Ig (same clone as 69002-1-PBS)

  Indirect ELISA was carried out by coating recombinant Human TNF Alpha (Cat.NO. HZ-1014) at 70 ng/well followed by blocking and adding serial diluted TNF Alpha antibody 69002-1-Ig and 69502-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm. This data was developed using the same antibody clone with 69002-1-PBS in a different storage buffer formulation.

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Product Information

69002-1-PBS targets NeutraKine® TNF-alpha in Neutralization, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: human Humankine TNF-alpha protein HZ-1014
• Full Name: tumor necrosis factor (TNF superfamily, member 2)
• Gene Symbol: TNF alpha
• Gene ID (NCBI): 7124
• Conjugate: Unconjugated
• Form: Lyophilized Powder
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Endotoxin: <0.1 EU/μg
• Reconstitution: This product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
• Stability and Storage: Lyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

Background Information

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, INS resistance, and cancer.

This antibody can be used to neutralize the bioactivity of TNF alpha.