Nesprin 3 Polyclonal antibody

Nesprin 3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

1

Reviews (1)

Cat no : 27132-1-AP

Synonyms

C14orf49, Nesprin 3

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Validation Data Gallery

• 

  WB analysis of C2C12 using 27132-1-AP

  C2C12 cell were subjected to SDS PAGE followed by western blot with 27132-1-AP (Nesprin 3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27132-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27132-1-AP (Nesprin 3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat spleen using 27132-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 27132-1-AP (Nesprin 3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 27132-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 27132-1-AP (Nesprin 3 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: C2C12 cells, rat spleen tissue, mouse spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

27132-1-AP targets Nesprin 3 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Nesprin 3 fusion protein Ag26002
• Full Name: chromosome 14 open reading frame 49
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: BC146604
• Gene Symbol: Nesprin 3
• Gene ID (NCBI): 161176
• RRID: AB_2880767
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nesprin 3 also known as C14orf49, SYNE3, belongs to the Nesprin family. Nesprin-3 is a type II transmembrane protein of the outer nuclear membrane and contains a C-terminal KASH domain and a series of spectrin repeats(PMID: 16330710). Nesprin-3, a protein that links intermediate filaments to the nucleus, regulates endothelial cell morphology, perinuclear cytoskeletal organization, and flow-induced cell polarization and migration. Nesprin-3 is robustly expressed in human aortic endothelial cells and localizes to the nuclear envelope(PMID: 21937718).

Publications

Species	Application	Title
mouse	IF	Front Cell Dev Biol; SUN5 Interacting With Nesprin3 Plays an Essential Role in Sperm Head-to-Tail Linkage: Research on Sun5 Gene Knockout Mice.; Authors - Yunfei Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Ning (Verified Customer) (01-28-2023)

Good antibody to prob nesprin 3.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse cortex lysate

Nesprin 3 Antibody Western Blot validation (1:2000 dilution) in mouse cortex lysate (Cat no:27132-1-AP)