Napsin A Polyclonal antibody, PBS Only

Napsin A Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 16558-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of mouse lung using 16558-1-AP (same clone as 16558-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16558-1-AP (Napsin A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16558-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse lung using 16558-1-AP (same clone as 16558-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16558-1-AP (Napsin A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16558-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse lung using 16558-1-AP (same clone as 16558-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16558-1-AP (Napsin A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16558-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 16558-1-AP (same clone as 16558-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16558-1-AP (Napsin A antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16558-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 16558-1-AP (same clone as 16558-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16558-1-AP (Napsin A antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16558-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 16558-1-AP (same clone as 16558-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16558-1-AP (Napsin A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16558-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 16558-1-AP (same clone as 16558-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16558-1-AP (Napsin A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16558-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 16558-1-AP (same clone as 16558-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16558-1-AP (Napsin A antibody at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16558-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 16558-1-AP (same clone as 16558-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16558-1-AP (Napsin A antibody at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16558-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HUVEC using 16558-1-AP (same clone as 16558-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using Napsin A antibody (16558-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16558-1-PBS in a different storage buffer formulation.

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Product Information

16558-1-PBS targets Napsin A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Napsin A fusion protein Ag9786
• Full Name: napsin A aspartic peptidase
• Calculated Molecular Weight: 420 aa, 45 kDa
• Observed Molecular Weight: 45-55 kDa
• GenBank Accession Number: BC017842
• Gene Symbol: Napsin A
• Gene ID (NCBI): 9476
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Napsin is found in two isoforms, napsin A and B, with highly homologous nucleotide sequences (91.2%). Napsin A appears to be a functional proteinase, predominantly expressed in lung and kidney. Napsin B is transcribed exclusively in cells related to the immune system and lacks an in-frame stop codon and is believed to be a pseudogene.(PMID:12698189). Napsin A is superior to TTF-1 in distinguishing primary lung ACA from other carcinomas (except kidney), particularly primary lung small cell carcinoma, and primary thyroid carcinoma.(PMID:22288963).