ChromoTek Nano-CaptureLigand® mouse IgE, VHH, biotinylated

The Nano-CaptureLigand® mouse IgE for the site-directed and specific immobilization of mouse IgE antibodies to avidin/streptavidin coated surfaces used in biosensor and ELISA assays. It consists of a biotinylated Nanobody that specifically binds to the mouse IgE subclass with high affinity and in a site-directed manner.

Applications

BLI, SPR, ELISA

Conjugate

Biotin

Host

Alpaca

Reactivity

Mouse IgE

Cat no : smsEB-1

Synonyms

Nano-CaptureLigand® mouse IgE, mouse IgE, mouse IgE VHH, immunoglobulin epsilon

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Validation Data Gallery

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  BLI binding kinetics of a mouse IgE antibody. 20 nM mouse IgE antibody was immobilized using Nano-CaptureLigand mouse IgE, VHH, biotinylated on FortéBio Streptavidin (SA) Biosensors. Mouse IgE is stably captured by the Nano-CaptureLigand with negligible dissociation.

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  ELISA capture of mouse IgE antibody using Nano-CaptureLigand mouse IgE, VHH, biotinylated. 50 nM Nano-CaptureLigand mouse IgE, VHH, biotinylated was used for coating on an avidin-coated MaxiSorp plate. Mouse IgE antibody was titrated in a 1:2 dilution series and detected with an alkaline phosphatase-conjugated detection antibody.

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  Immobilization of the Nano-CaptureLigand (green) on Biosensor (white) for biolayer interferometry (BLI) assay followed by capture of an antibody (orange). The setup is ready for characterization of the antibodies’ binding kinetics and affinity to target (red), antibody discovery and screening in crude liquids like hybridoma supernatant, serum, and plasma.

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  BLI binding kinetics of mouse, human and rabbit antibodies. 20 nM of mouse, human and rabbit antibodies were immobilized using Nano-CaptureLigand mouse IgE, VHH, biotinylated on FortéBio Streptavidin (SA) Biosensors. Only mouse IgE antibody is captured by the Nano-CaptureLigand with negligible dissociation.

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  Sandwich ELISA: Immobilization of the Nano-CaptureLigand on streptavidin coated microplates for ELISA. No biotinylation of the antibody is required. Coating of the streptavidin microplate with Nano-CaptureLigand (top left). Site-directed antibody immobilization via Nano-CaptureLigand (top right). Addition of sample including target (red, bottom left). Addition of detection and secondary antibody for read-out (bottom right).

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  Species reactivity of Secondary VHH.

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Product Information

The Nano-CaptureLigand® mouse IgE for the site-directed and specific immobilization of mouse IgE antibodies to avidin/streptavidin coated surfaces used in biosensor and ELISA assays. It consists of a biotinylated Nanobody that specifically binds to the mouse IgE subclass with high affinity and in a site-directed manner.

• Description: The next level of antibody immobilization to BLI and SPR biosensors

  • Gentle, site-directed immobilization of mouse IgE antibodies to streptavidin coated biosensors without biotinylation of the antibody

  • Compatible to surface plasmon resonance (SPR) and biolayer interferometry (BLI) assays (e.g. FortéBio Octet® systems)

  • High affinity for stable baseline without significant dissociation

  • >10x regeneration for multiple reuse

  • High specificity enables mouse IgE antibody discovery and screening in crude liquids like hybridoma supernatant, serum, and plasma samples

• Applications: BLI, SPR, ELISA
• Host: Alpaca
• Reactivity: Mouse IgE
• Conjugate: Biotin
• Physical State: Liquid
• Purification Method: Recombinant expression, affinity purification IMAC
• Type: Nanobody
• Class: Recombinant
• Affinity (KD): K_D =7.80 nM; Apparent affinity may be higher for full IgEs due to avidity effects (1 antibody captured by 2 Nanobodies).
• Storage Buffer: 25 mM TAPS pH 8.5, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % sodium azide
• Storage Condition: Shipped at ambient temperature. Upon receipt store at +4°C/+40°F. Optional: Aliquot upon arrival and store at -20°C/-4°F
• RRID: AB_2864257
• Note: This product is for research use only, not for diagnostic or therapeutic use