Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, Jurkat cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human prostate cancer tissue, human ovary cancer tissue, human placenta tissue, human colon cancer tissue, human appendicitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12024-1-AP targets NUSAP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2654 Product name: Recombinant human NUSAP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-440 aa of BC001308 Sequence: MIIPSLEELDSLKYSDLQNLAKSLGLRANLRATKLLKALKGYIKHEARKGNENQDESQTSASSCDETEIQISNQEEAERQPLGHVTKTRRRCKTVRVDPDSQQNHSEIKISNPTEFQNHEKQESQDLRATAKVPSPPDEHQEAENAVSSGNRDSKVPSEGKKSLYTDESSKPGKNKRTAITTPNFKKLHEAHFKEMESIDQYIERKKKHFEEHNSMNELKQPINKGGVRTPVPPRGRLSVASTPISQRRSQGRSCGPASQSTLGLKGSLKRSAISAAKTGVRFSAATKDNEHKRSLTKTPARKSAHVTVSGGTPKGEAVLGTHKLKTITGNSAAVITPFKLTTEATQTPVSNKKPVFDLKASLSRPLNYEPHKGKLKPWGQSKENNYLNQHVNRINFYKKTYKQPHLQTKEEQRKKREQERKEKKAKVLGMRRGLILAED Predict reactive species |
| Full Name | nucleolar and spindle associated protein 1 |
| Calculated Molecular Weight | 440 aa, 49 kDa |
| Observed Molecular Weight | 49-60 kDa |
| GenBank Accession Number | BC001308 |
| Gene Symbol | NUSAP1 |
| Gene ID (NCBI) | 51203 |
| RRID | AB_2157789 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BXS6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NUSAP1, also named as ANKT, belongs to the NUSAP family. It is microtubule-associated protein with the capacity to bundle and stabilize microtubules. NUSAP1 may associate with chromosomes and promote the organization of mitotic spindle microtubules around them. NUSAP1 plays a role in connecting apoptosis with cell migration and it is a potentially involved in morphogenesis in vertebrates (PMID:21203972). NUSAP1 is an immunogenic antigen in 65% of patients with AML following allogeneic HCT and suggests a tumor antigen role(PMID:20053754 ). NuSAP1 protein level is tightly regulated by the APC/C ubiquitin ligase complex and NuSAP induces mitotic arrest dependent of its microtubule affinity (PMID:17618083). NUSAP1 is overexpressed in pituitary adenomas.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NUSAP1 antibody 12024-1-AP | Download protocol |
| IHC protocol for NUSAP1 antibody 12024-1-AP | Download protocol |
| IP protocol for NUSAP1 antibody 12024-1-AP | Download protocol |
| WB protocol for NUSAP1 antibody 12024-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-NUSAP1 antibody (Cat# 12024-1-AP) has accumulated 55 citations in high-impact journals including Science, Cell, Nature Communications, Molecular Cell, Gastroenterology, Advanced Science, Oncogene, Journal of Experimental Medicine, and Acta Neuropathologica. Research fields span oncology (glioblastoma, prostate, liver, lung, breast, gastric, pancreatic, ovarian, bladder, esophageal, and thyroid cancers), cell division and mitotic regulation, oocyte maturation, protein degradation pathways, and metabolic signaling.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther NUSAP1 potentiates chemoresistance in glioblastoma through its SAP domain to stabilize ATR. | ||
Gastroenterology microRNA 193a-5p Regulates Levels of Nucleolar- and Spindle-Associated Protein 1 to Suppress Hepatocarcinogenesis. | ||
Nat Commun YY1 enhances HIF-1α stability in tumor-associated macrophages to suppress anti-tumor immunity of prostate cancer in mice
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