NUDT9 Polyclonal antibody

NUDT9 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 15068-1-AP

Synonyms

ADP ribose diphosphatase, ADP ribose phosphohydrolase, ADPR PPase, Nudix motif 9, NUDT10, NUDT9, UNQ3012/PRO9771

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Validation Data Gallery

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  WB analysis using 15068-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15068-1-AP (NUDT9 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 15068-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 15068-1-AP (NUDT9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 15068-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15068-1-AP (NUDT9 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 15068-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15068-1-AP (NUDT9 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 15068-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15068-1-AP (NUDT9 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 15068-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15068-1-AP (NUDT9 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, Raji cells, rat brain tissue
• Positive IHC detected in: human heart tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

15068-1-AP targets NUDT9 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NUDT9 fusion protein Ag7075
• Full Name: nudix (nucleoside diphosphate linked moiety X)-type motif 9
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC000542
• Gene Symbol: NUDT9
• Gene ID (NCBI): 53343
• RRID: AB_10951855
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Sci Rep; Quantitative proteomics by SWATH-MS reveals sophisticated metabolic reprogramming in hepatocellular carcinoma tissues.; Authors - Yanyan Gao
rat	WB	Sci Rep; Involvement of TRPM2 and TRPV1 channels on hyperalgesia, apoptosis and oxidative stress in rat fibromyalgia model: Protective role of selenium.; Authors - Esra Yüksel