NTM Polyclonal antibody

• Featured Product
• KD/KO Validated

NTM Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14373-1-AP

Synonyms

HNT, IgLON family member 2, IGLON2, neurotrimin, NT, NTM, NTRI

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Validation Data Gallery

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  WB analysis of SH-SY5Y using 14373-1-AP

  WB result of NTM antibody (14373-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NTM transfected SH-SY5Y cells.

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  WB analysis of SH-SY5Y using 14373-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14373-1-AP (NTM Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14373-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14373-1-AP (NTM Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 14373-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14373-1-AP (NTM antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 14373-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14373-1-AP (NTM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, mouse brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14373-1-AP targets NTM in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NTM fusion protein Ag5746
• Full Name: neurotrimin
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 58 kDa, 65 kDa
• GenBank Accession Number: BC050716
• Gene Symbol: NTM
• Gene ID (NCBI): 50863
• RRID: AB_2878048
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Neurotrimin (NTM) is a glycosylphosphatidylinositol (GPI)-anchored neural cell adhesion molecule. Neurotrimin together with the limbic system-associated protein (LAMP) and the opioid binding cell adhesion molecule (OBCAM) comprise the IgLON family of the immunoglobulin superfamily. Neurotrimin may be promoting neurite outgrowth and adhesion via a homophilic mechanism.