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  • Featured Product
  • KD/KO Validated

NRF2, NFE2L2 Monoclonal antibody, PBS Only (Detector)

NRF2, NFE2L2 Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E9E3

Cat no : 66504-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using NRF2, NFE2L2 Monoclonal antibody (66504-1-Ig)

    WB analysis of HeLa using 66504-1-Ig (same clone as 66504-1-PBS)

    Untreated and MG132 treated Hela cells were subjected to SDS PAGE followed by western blot with 66504-1-Ig (NRF2, NFE2L2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66504-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50316-2

    Cytometric bead array standard curve of MP50316-2

    Cytometric bead array standard curve of MP50316-2, NRF2, NFE2L2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66504-2-PBS. Detection antibody: 66504-1-PBS. Standard:Ag9469. Range: 1.563-100 ng/mL.

Product Information

66504-1-PBS targets NRF2, NFE2L2 as part of a matched antibody pair:

MP50316-2: 66504-2-PBS capture and 66504-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen NRF2, NFE2L2 fusion protein Ag9469
Full Name nuclear factor (erythroid-derived 2)-like 2
Calculated Molecular Weight 605 aa, 68 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC011558
Gene Symbol NRF2
Gene ID (NCBI) 4780
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

NRF2, also named as NFE2L2, belongs to the bZIP family and CNC subfamily. It is a transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. NRF2 is important for the coordinated up-regulation of genes in response to oxidative stress. It may be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. Nrf2 is a key player in the regulation of genes encoding for many antioxidative response enzymes.The expression of NRF2 may be induced under oxidative stress (PMID:14567983).In lung cancer, Nrf2 activation in malignant cells has been associated with tumor progression and chemotherapy resistance(PMID:20534738). Identifying patients with abnormal NRF2 expression may be important for selection for chemotherapy in NSCLC. As new investigators break into the emerging field of Nrf2 research, confusion regarding the correct migratory pattern of Nrf2 is causing doubts about the accuracy and reproducibility of published results. This letter provides solid evidence that the actually observed molecular weight of Nrf2 is about 70kDa and 95-110 kDa. (PMID: 22703241).