NRAS-Specific Polyclonal antibody

NRAS-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18296-1-AP

Synonyms

ALPS4, GTPase NRas, HRAS1, N ras, NRAS, NRAS1, NRAS-Specific, Transforming protein N Ras

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Validation Data Gallery

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  WB analysis using 18296-1-AP

  Recombinant fusion protein Ag27444 (KRAS-2A), Ag21645(KRAS-2B), Ag29746 (NRAS), and Ag23265(HRAS) were subjected to SDS PAGE followed by western blot with 18296-1-AP (NRAS-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 18296-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18296-1-AP (NRAS-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 18296-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 18296-1-AP (NRAS-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 18296-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 18296-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human malignant melanoma using 18296-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 18296-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 18296-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 18296-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 18296-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 18296-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 18296-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat colon using 18296-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 18296-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 18296-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 18296-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human malignant melanoma using 18296-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A431 cells, MCF-7 cells
• Positive IHC detected in: human colon tissue, human kidney tissue, human colon cancer tissue, rat colon tissue, mouse colon tissue, human malignant melanoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18296-1-AP targets NRAS-Specific in WB, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.

• Tested Reactivity: human, rat, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: neuroblastoma RAS viral (v-ras) oncogene homolog
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC005219
• Gene Symbol: NRAS
• Gene ID (NCBI): 4893
• RRID: AB_2918061
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NRAS, also named as N-ras and NRAS1, is neuroblastoma RAS viral (v-ras) oncogene homolog from the mammalian ras gene family and it is a member of the small GTPase superfamily. RAS proteins are involved in signal transduction pathways, and bind GDP/GTP and possess intrinsic GTPase activity. It is mapped on chromosome 1, and it is activated in HL60, a promyelocytic leukemia line. Defects in NRAS are a cause of juvenile myelomonocytic leukemia (JMML). The antibody is specific to NRAS, can't recognize HRAS and KRAS.