NR2F1 Polyclonal antibody

NR2F1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

4

Reviews (1)

Cat no : 24573-1-AP

Synonyms

COUP TF I, COUP TF1, COUP TFI, COUP transcription factor 1, COUP transcription factor I, EAR 3, EAR3, ERBAL3, NR2F1, NR2F2, SVP44, TCFCOUP1, TFCOUP1, V erbA related protein 3

---

Validation Data Gallery

• 

  WB analysis using 24573-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24573-1-AP (NR2F1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary using 24573-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 24573-1-AP (NR2F1 Antibody) at dilution of 1:50 (under 40x lens).

---

Tested Applications

• Positive WB detected in: HEK-293 cells, PC-3 cells, NIH/3T3 cells
• Positive IHC detected in: human ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

24573-1-AP targets NR2F1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NR2F1 fusion protein Ag0118
• Full Name: nuclear receptor subfamily 2, group F, member 1
• Calculated molecular weight: 50 kDa
• Observed molecular weight: 46 kDa
• GenBank accession number: BC004154
• Gene symbol: NR2F1
• Gene ID (NCBI): 7025
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NR2F1 (Nuclear receptor subfamily 2, group F, member 1), also known as COUP-TFI, is an orphan nuclear receptor belonging to the superfamily of steroid/thyroid hormone receptors and acting as a strong transcriptional regulator. NR2F1 has a DNA binding domain (DBD) consisting of two conserved Zinc-finger motifs and a ligand-binding domain (LBD). NR2F1 also plays an epigenetic role in mediating global histone modifications by interacting with or recruiting chromatin-remodeling enzymes. NR2F1 can be a biomarker of tumor dormancy that promotes quiescence of various types of cancer cells, including breast, prostate, and squamous cell carcinoma of the head and neck (PMID: 35740627).

Publications

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
human	IHC	J Exp Clin Cancer Res; Inhibition of DEC2 is necessary for exiting cell dormancy in salivary adenoid cystic carcinoma.; Authors - Xiao Yang
human	WB,IF	Cell Death Dis; Nanog mediated by FAO/ACLY signaling induces cellular dormancy in colorectal cancer cells.; Authors - Meng Zhang
human	WB,IHC	Cancers (Basel); NR2F1 Regulates TGF-β1-Mediated Epithelial-Mesenchymal Transition Affecting Platinum Sensitivity and Immune Response in Ovarian Cancer; Authors - Qiuju Liang ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Ryan (Verified Customer) (06-15-2018)

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: zebrafish retina

NR2F1 Antibody Immunohistochemistry validation (1:100 dilution) in zebrafish retina (Cat no:24573-1-AP)