|Positive WB detected in||HEK-293 cells, PC-3 cells, NIH/3T3 cells|
|Positive IHC detected in||human ovary tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
24573-1-AP targets NR2F1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||NR2F1 fusion protein Ag0118|
|Full Name||nuclear receptor subfamily 2, group F, member 1|
|Calculated molecular weight||50 kDa|
|Observed molecular weight||46 kDa|
|GenBank accession number||BC004154|
|Gene ID (NCBI)||7025|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
NR2F1 (Nuclear receptor subfamily 2, group F, member 1), also known as COUP-TFI, is an orphan nuclear receptor belonging to the superfamily of steroid/thyroid hormone receptors and acting as a strong transcriptional regulator. NR2F1 has a DNA binding domain (DBD) consisting of two conserved Zinc-finger motifs and a ligand-binding domain (LBD). NR2F1 also plays an epigenetic role in mediating global histone modifications by interacting with or recruiting chromatin-remodeling enzymes. NR2F1 can be a biomarker of tumor dormancy that promotes quiescence of various types of cancer cells, including breast, prostate, and squamous cell carcinoma of the head and neck (PMID: 35740627).
Divergent transcriptional regulation of astrocyte reactivity across disorders.
J Exp Clin Cancer Res
Inhibition of DEC2 is necessary for exiting cell dormancy in salivary adenoid cystic carcinoma.
Cell Death Dis
Nanog mediated by FAO/ACLY signaling induces cellular dormancy in colorectal cancer cells.
NR2F1 Regulates TGF-β1-Mediated Epithelial-Mesenchymal Transition Affecting Platinum Sensitivity and Immune Response in Ovarian Cancer
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Ryan (Verified Customer) (06-15-2018)