NR2F1 Polyclonal antibody

• Featured Product
• KD/KO Validated

NR2F1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

7

Reviews (1)

Cat no : 24573-1-AP

Synonyms

COUP TF I, COUP TF1, COUP TFI, COUP transcription factor 1, COUP transcription factor I, EAR 3, EAR3, ERBAL3, NR2F1, NR2F2, SVP44, TCFCOUP1, TFCOUP1, V erbA related protein 3

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Validation Data Gallery

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  WB analysis of HEK-293 using 24573-1-AP

  WB result of NR2F1 antibody (24573-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-NR2F1 transfected HEK-293 cells.

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  WB analysis using 24573-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24573-1-AP (NR2F1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 24573-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24573-1-AP (NR2F1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary using 24573-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 24573-1-AP (NR2F1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 24573-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 24573-1-AP (NR2F1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human stomach cancer using 24573-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24573-1-AP (NR2F1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, PC-3 cells, NIH/3T3 cells
• Positive IHC detected in: human stomach cancer tissue, human ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

24573-1-AP targets NR2F1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NR2F1 fusion protein Ag0118
• Full Name: nuclear receptor subfamily 2, group F, member 1
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC004154
• Gene Symbol: NR2F1
• Gene ID (NCBI): 7025
• RRID: AB_2879616
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NR2F1 (Nuclear receptor subfamily 2, group F, member 1), also known as COUP-TFI, is an orphan nuclear receptor belonging to the superfamily of steroid/thyroid hormone receptors and acting as a strong transcriptional regulator. NR2F1 has a DNA binding domain (DBD) consisting of two conserved Zinc-finger motifs and a ligand-binding domain (LBD). NR2F1 also plays an epigenetic role in mediating global histone modifications by interacting with or recruiting chromatin-remodeling enzymes. NR2F1 can be a biomarker of tumor dormancy that promotes quiescence of various types of cancer cells, including breast, prostate, and squamous cell carcinoma of the head and neck (PMID: 35740627).

Publications

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
human	IHC	J Exp Clin Cancer Res; Inhibition of DEC2 is necessary for exiting cell dormancy in salivary adenoid cystic carcinoma.; Authors - Xiao Yang
human	WB,IF	Cell Death Dis; Nanog mediated by FAO/ACLY signaling induces cellular dormancy in colorectal cancer cells.; Authors - Meng Zhang
human	WB,IHC	Cancers (Basel); NR2F1 Regulates TGF-β1-Mediated Epithelial-Mesenchymal Transition Affecting Platinum Sensitivity and Immune Response in Ovarian Cancer; Authors - Qiuju Liang ; KD Validated
human	WB	Clin Transl Med; Cullin-associated and neddylation-dissociated 1 regulate reprogramming of lipid metabolism through SKP1-Cullin-1-F-boxFBXO11 -mediated heterogeneous nuclear ribonucleoprotein A2/B1 ubiquitination and promote hepatocellular carcinoma; Authors - Hao Zhang
human	WB	Transl Oncol; Hypoxia induced cell dormancy of salivary adenoid cystic carcinoma through miR-922/DEC2 axis; Authors - Li Dai

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Ryan (Verified Customer) (06-15-2018)

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: zebrafish retina

NR2F1 Antibody Immunohistochemistry validation (1:100 dilution) in zebrafish retina (Cat no:24573-1-AP)