NR2F1 Polyclonal antibody

NR2F1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 24573-1-AP

Synonyms

COUP TF I, COUP TF1, COUP TFI, COUP transcription factor 1, COUP transcription factor I, EAR 3, EAR3, ERBAL3, NR2F1, NR2F2, SVP44, TCFCOUP1, TFCOUP1, V erbA related protein 3



Tested Applications

Positive WB detected inHEK-293 cells, PC-3 cells, NIH/3T3 cells
Positive IHC detected inhuman ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

24573-1-AP targets NR2F1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NR2F1 fusion protein Ag0118
Full Name nuclear receptor subfamily 2, group F, member 1
Calculated molecular weight 50 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC004154
Gene symbol NR2F1
Gene ID (NCBI) 7025
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NR2F1 (Nuclear receptor subfamily 2, group F, member 1), also known as COUP-TFI, is an orphan nuclear receptor belonging to the superfamily of steroid/thyroid hormone receptors and acting as a strong transcriptional regulator. NR2F1 has a DNA binding domain (DBD) consisting of two conserved Zinc-finger motifs and a ligand-binding domain (LBD). NR2F1 also plays an epigenetic role in mediating global histone modifications by interacting with or recruiting chromatin-remodeling enzymes. NR2F1 can be a biomarker of tumor dormancy that promotes quiescence of various types of cancer cells, including breast, prostate, and squamous cell carcinoma of the head and neck (PMID: 35740627).

Protocols

Product Specific Protocols
WB protocol for NR2F1 antibody 24573-1-APDownload protocol
IHC protocol for NR2F1 antibody 24573-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Nature

Divergent transcriptional regulation of astrocyte reactivity across disorders.

Authors - Joshua E Burda
humanIHC

J Exp Clin Cancer Res

Inhibition of DEC2 is necessary for exiting cell dormancy in salivary adenoid cystic carcinoma.

Authors - Xiao Yang
humanWB,IF

Cell Death Dis

Nanog mediated by FAO/ACLY signaling induces cellular dormancy in colorectal cancer cells.

Authors - Meng Zhang
humanWB,IHC

Cancers (Basel)

NR2F1 Regulates TGF-β1-Mediated Epithelial-Mesenchymal Transition Affecting Platinum Sensitivity and Immune Response in Ovarian Cancer

Authors - Qiuju Liang
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Ryan (Verified Customer) (06-15-2018)

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: zebrafish retina
NR2F1 Antibody Immunohistochemistry validation (1:100 dilution) in zebrafish retina (Cat no:24573-1-AP)