NPHP5/IQCB1 Polyclonal antibody

NPHP5/IQCB1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 15747-1-AP

Synonyms

IQ motif containing B1, IQCB1, KIAA0036, Nephrocystin 5, NPHP5, NPHP5,IQCB1, PIQ, SLSN5

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Validation Data Gallery

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  WB analysis using 15747-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15747-1-AP (NPHP5,IQCB1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

15747-1-AP targets NPHP5/IQCB1 in WB, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NPHP5/IQCB1 fusion protein Ag8203
• Full Name: IQ motif containing B1
• Calculated Molecular Weight: 598 aa, 69 kDa
• Observed Molecular Weight: 69 kDa
• GenBank Accession Number: BC005806
• Gene Symbol: IQCB1
• Gene ID (NCBI): 9657
• RRID: AB_2878179
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IQCB1, also known as NPHP5, is a nephrocystin protein that interacts with calmodulin and the retinitis pigmentosa GTPase regulator protein. It has a central coiled-coil region and two calmodulin-binding IQ domains. Localized to the primary cilia of renal epithelial cells and connecting cilia of photoreceptor cells, IQCB1 is thought to play a role in ciliary function. Mutations in this gene result in Senior-Loken syndrome type 5, a juvenile disorder characterized by defects in the waste filtering system of the kidney, as well as retinal degradation.

Publications

Species	Application	Title
human	IF	Hum Mol Genet; Pathogenic NPHP5 mutations impair protein interaction with Cep290, a prerequisite for ciliogenesis.; Authors - Barbelanne Marine M
human		Mol Biol Cell; Rpgrip1l controls ciliary gating by ensuring the proper amount of Cep290 at the vertebrate transition zone.; Authors - Antonia Wiegering