NOX4 Polyclonal antibody

• Featured Product
• KD/KO Validated

NOX4 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

284

Reviews (1)

Cat no : 14347-1-AP

Synonyms

NOX4 Rabbit PolyAb, KOX 1, KOX, Kidney superoxide-producing NADPH oxidase, Kidney oxidase-1

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Validation Data Gallery

• 

  WB analysis using 14347-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14347-1-AP (NOX4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14347-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14347-1-AP (NOX4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14347-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14347-1-AP (NOX4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 14347-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14347-1-AP (NOX4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 14347-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14347-1-AP (NOX4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of BxPC-3 using 14347-1-AP

  Immunofluorescent analysis of BxPC-3 cells using 14347-1-AP (NOX4 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 14347-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human NOX4 (14347-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Tested Applications

• Positive WB detected in: BxPC-3 cells, HeLa cells, HEK-293 cells, mouse kidney tissue, JAR cells, mouse lung tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: BxPC-3 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 13 publications below
• WB: See 250 publications below
• IHC: See 46 publications below
• IF: See 25 publications below
• IP: See 2 publications below
• FC: See 2 publications below
• CoIP: See 2 publications below

Product Information

14347-1-AP targets NOX4 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NOX4 fusion protein Ag5687
• Full Name: NADPH oxidase 4
• Calculated Molecular Weight: 67 kDa
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: BC040105
• Gene Symbol: NOX4
• Gene ID (NCBI): 50507
• RRID: AB_10638146
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NOX4 (NADPH oxidase 4) is a phagocyte-type oxidase, similar to that responsible for the production of large amounts of reactive oxygen species (ROS) in neutrophil granulocytes with resultant antimicrobial activity and it has been postulated to function in the kidney as an oxygen sensor that regulates the synthesis of erythropoietin in the renal cortex. Studies have reported molecular masses of Nox4 protein by western blot analysis ranging from 55 to 80 kDa. The truncated NOX4 splice variant D (28 kDa) lacks the majority of the transmembrane domain and has been shown to produce higher levels of ROS and DNA damage compared to its prototype. NOX4D has previously been shown to localise to the nucleus and nucleolus in various cell types and is implicated in the generation of reactive oxygen species (ROS) and DNA damage. (PMID: 11728818, PMID: 29285262, PMID: 14670934)

Publications

Species	Application	Title
mouse	WB	Circulation; Megakaryocytic Leukemia 1 (MKL1) Bridges Epigenetic Activation of NADPH Oxidase in Macrophages to Cardiac Ischemia-Reperfusion Injury.; Authors - Yu Liming
		Nat Commun; Spatial oxidation of L-plastin downmodulates actin-based functions of tumor cells.; Authors - Emre Balta
mouse	WB	Aging Cell; Lysyl oxidase-like 2 inhibitor rescues D-galactose-induced skeletal muscle fibrosis.; Authors - Yongxin Wu
mouse	WB	Adv Healthc Mater; Copper-Based Composites Nanoparticles Improve Triple-Negative Breast Cancer Treatment with Induction of Apoptosis-Cuproptosis and Immune Activation; Authors - Ning Wang
human	WB,IF	Redox Biol; Silencing COX-2 blocks PDK1/TRAF4-induced AKT activation to inhibit fibrogenesis during skeletal muscle atrophy.; Authors - Hongtao Chen
rat	WB	Redox Biol; RND3 attenuates oxidative stress and vascular remodeling in spontaneously hypertensive rat via inhibiting ROCK1 signaling.; Authors - Nan Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

K (Verified Customer) (02-02-2022)

This antibody gave us really nice bands in 1:1000 dilution with 5% BSA+TBST.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Hela

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