nNOS Polyclonal antibody

nNOS Polyclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


Mouse, Rat, Human and More (1)





Cat no : 29231-1-AP


bNOS, Constitutive NOS, IHPS1, N NOS, NC NOS, Neuronal NOS, Nitric oxide synthase, brain, nNOS, NOS, NOS type I, NOS1

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 2 publications below

Product Information

29231-1-AP targets nNOS in WB, IF, FC, IHC, ELISA applications and shows reactivity with Mouse, Rat, Human samples.

Tested Reactivity Mouse, Rat, Human
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen nNOS fusion protein Ag28910
Full Name nitric oxide synthase 1 (neuronal)
Calculated Molecular Weight 161 kDa
Observed Molecular Weight160 kDa
GenBank Accession NumberNM_000620
Gene Symbol NOS1
Gene ID (NCBI) 4842
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

nNOS belongs to the NOS family. nNOS produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. NO is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. NO is synthesized from L-arginine by nitric oxide synthases. nNOS is a nitric oxide synthase which is highly expressed in skeletal muscle. Genetic variations in nNOS gene are associated with susceptibility to infantile hypertrophic pyloric stenosis type 1 (IHPS1).The antibody can recognize isoform 1,2,4 of nNOS.


Product Specific Protocols
WB protocol for nNOS antibody 29231-1-APDownload protocol
IHC protocol for nNOS antibody 29231-1-APDownload protocol
IF protocol for nNOS antibody 29231-1-APDownload protocol
FC protocol for nNOS antibody 29231-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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