Tested Applications
| Positive IHC detected in | human pancreas cancer tissue, human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12256-1-AP targets NLRP1 in IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2908 Product name: Recombinant human NLRP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-146 aa of BC016965 Sequence: MPLDPYHSVTWGHAQGSHHFVFGELRVRPILESLRDEPDPDPRPSREGPAGRVGALARGGPEPCDAASPPGGASCAPELARPREDKSAQQAKLEGGTRLCCRCPEESRLVPGGAVSPGDHVLEVSGTRGTCGCRPRRHAGPELAHS Predict reactive species |
| Full Name | NLR family, pyrin domain containing 1 |
| Calculated Molecular Weight | 155 kDa |
| GenBank Accession Number | BC016965 |
| Gene Symbol | NLRP1 |
| Gene ID (NCBI) | 22861 |
| RRID | AB_2298504 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9C000 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for NLRP1 antibody 12256-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NLRP1 antibody (Cat# 12256-1-AP) has 20 citations across journals including Immunity, Nature Communications, Molecular Neurodegeneration, Journal of Neuroinflammation, Frontiers in Immunology, PLoS Pathogens, Scientific Reports, and iScience. Research fields span neurodegeneration (Alzheimer's, Parkinson's), inflammasome signaling, pyroptosis, autoimmune thyroiditis, ischemic stroke, endometriosis, infectious diseases, ulcerative colitis, lung adenocarcinoma, and dermatology (SJS/TEN).
| Species | Application | Title |
|---|---|---|
Nat Commun Schwann cell C5aR1 co-opts inflammasome NLRP1 to sustain pain in a mouse model of endometriosis | ||
J Neuroinflammation Targeting CB2R in astrocytes for Parkinson's disease therapy: unraveling the Foxg1-mediated neuroprotective mechanism through autophagy-mediated NLRP3 degradation | ||
Front Immunol Cytokine Secretion and Pyroptosis of Thyroid Follicular Cells Mediated by Enhanced NLRP3, NLRP1, NLRC4, and AIM2 Inflammasomes Are Associated With Autoimmune Thyroiditis. | ||
NanoImpact Protein corona exacerbated inflammatory response in macrophages elicited by CdTe quantum dots |
Reviews
What customers say
The single review for 12256-1-AP is a 5-star rating from a user who performed Western Blot on human fibroblasts at a 1:250 dilution. They reported that the band was very clear and of exact molecular weight, indicating high specificity and reliability for their application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Debanjan (Verified Customer) (08-17-2020) | The band was very clear and of exact molecular weight
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