IkB Alpha Polyclonal antibody

IkB Alpha Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 51066-1-AP

Synonyms

I kappa B alpha, IkappaBalpha, IkB alpha, IKBA, MAD 3, MAD3, NF kappa B inhibitor alpha, NFKBI, NFKBIA

---

Validation Data Gallery

• 

  WB analysis of PC-3 using 51066-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 51066-1-AP (IkB alpha antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 51066-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 51066-1-AP (IkB alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 51066-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 51066-1-AP (IkB alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 51066-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 51066-1-AP (IkB alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 51066-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 51066-1-AP (IkB alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 51066-1-AP

  IP result of anti-IkB Alpha (IP:51066-1-AP, 4ug; Detection:51066-1-AP 1:1000) with HeLa cells lysate 3200ug.

• 

  IHC staining of human cervical cancer using 51066-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 51066-1-AP (IkB alpha antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 51066-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 51066-1-AP (IkB alpha antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 51066-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 51066-1-AP (IkB alpha antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 51066-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 51066-1-AP (IkB alpha antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 51066-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 51066-1-AP (IkB alpha antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 51066-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 51066-1-AP (IkB alpha antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human cervical cancer using 51066-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 51066-1-AP (IkB alpha antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human cervical cancer using 51066-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 51066-1-AP (IkB alpha antibody) at dilution of 1:100 (under 10x lens).

---

Tested Applications

• Positive WB detected in: PC-3 cells, HeLa cells, Jurkat cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human cervical cancer tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 14 publications below

Product Information

51066-1-AP targets IkB Alpha in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC002601
• Gene Symbol: IkB Alpha
• Gene ID (NCBI): 4792
• RRID: AB_2235946
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime.

Publications

Species	Application	Title
	WB	J Hazard Mater; Melatonin alleviates Ochratoxin A-induced liver inflammation involved intestinal microbiota homeostasis and microbiota-independent manner.; Authors - Daiyang Xia
human	WB	J Immunol; Enterovirus 71 2C protein inhibits TNF-α-mediated activation of NF-κB by suppressing IκB kinase β phosphorylation.; Authors - Zheng Zhenhua Z
mouse	WB	Front Immunol; Aspartate Metabolism Facilitates IL-1β Production in Inflammatory Macrophages.; Authors - Hao Wang
mouse	WB	Front Immunol; Serine Supports IL-1β Production in Macrophages Through mTOR Signaling.; Authors - Siyuan Chen
mouse	WB	FASEB J; Nintedanib ameliorates osteoarthritis in mice by inhibiting synovial inflammation and fibrosis caused by M1 polarization of synovial macrophages via the MAPK/PI3K-AKT pathway; Authors - Jiangbo Yan
mouse	WB	Nutrients; Fish Hydrolysate Supplementation Containing n-3 Long Chain Polyunsaturated Fatty Acids and Peptides Prevents LPS-Induced Neuroinflammation.; Authors - Mathilde Chataigner