NFKB2,p52,p100-Specific Polyclonal antibody

• Featured Product
• KD/KO Validated

NFKB2,p52,p100-Specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 15503-1-AP

Synonyms

NFKB2, NF kappa B, LYT10, LYT 10, H2TF1

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Validation Data Gallery

• 

  WB analysis of A431 using 15503-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 15503-1-AP (NFKB2,p52,p100-Specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15503-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15503-1-AP (NFKB2,p52,p100-Specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 15503-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 15503-1-AP (NFKB2,p52,p100-Specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 15503-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15503-1-AP (NFKB2,p52,p100-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human spleen using 15503-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15503-1-AP (NFKB2,p52,p100-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 15503-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15503-1-AP (NFKB2,p52,p100-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 15503-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15503-1-AP (NFKB2,p52,p100-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 15503-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15503-1-AP (NFKB2,p52,p100-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 15503-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15503-1-AP (NFKB2,p52,p100-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 15503-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15503-1-AP (NFKB2,p52,p100-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 15503-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15503-1-AP (NFKB2,p52,p100-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 15503-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15503-1-AP (NFKB2,p52,p100-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 15503-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15503-1-AP (NFKB2,p52,p100-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 15503-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15503-1-AP (NFKB2,p52,p100-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 15503-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15503-1-AP (NFKB2,p52,p100-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 15503-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15503-1-AP (NFKB2,p52,p100-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 15503-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15503-1-AP (NFKB2,p52,p100-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 15503-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15503-1-AP (NFKB2,p52,p100-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 15503-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NFKB2,p52,p100-Specific antibody (15503-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), 有核膜 (red).

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, MCF-7 cells, mouse heart tissue
• Positive IHC detected in: human testis tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

15503-1-AP targets NFKB2,p52,p100-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)
• Calculated Molecular Weight: 97 kDa
• Observed Molecular Weight: 52/100 kDa
• GenBank Accession Number: NM_001077494
• Gene Symbol: NFKB2
• Gene ID (NCBI): 4791
• RRID: AB_2267126
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. P52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. P52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. This antibody can bind both p52 and p100 isoforms of NFKB2.

Publications

Species	Application	Title
human	IHC	Cancers (Basel); Identification of Prognostic Markers of Gynecologic Cancers Utilizing Patient-Derived Xenograft Mouse Models.; Authors - Ha-Yeon Shin
human	IF,WB	Sci Rep; TNFR2 ligation in human T regulatory cells enhances IL2-induced cell proliferation through the non-canonical NF-κB pathway.; Authors - Jun Wang
human	WB	Pharm Biol; Simiao Qingwen Baidu decoction inhibits Epstein-Barr virus-induced B lymphoproliferative disease and lytic viral replication.; Authors - Xianhui Yang
human	WB	Mol Cell Endocrinol; Interleukin-1 increases cyclooxygenase-2 expression and prostaglandin E2 production in human granulosa-lutein cell via nuclear factor kappa B/P65 and extracellular signal-regulated kinase 1/2 signaling pathways; Authors - Shan Wan ; KD Validated
human	WB	Theranostics; Platelet membrane-coated alterbrassicene A nanoparticle inhibits calcification of the aortic valve by suppressing phosphorylation P65 NF-κB; Authors - Bingchuan Geng
human	WB	Oncogene; Monoacylglycerol lipase blockades the senescence-associated secretory phenotype by interfering with NF-κB activation and promotes docetaxel efficacy in prostate cancer; Authors - Jianpeng Yu