NFATC3 Monoclonal antibody

NFATC3 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1C1H8

Cat no : 66924-1-Ig

Synonyms

NF AT4, NF ATc3, NFAT4, NFATC3, NFATX

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Validation Data Gallery

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  WB analysis using 66924-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66924-1-Ig (NFATC3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human thymus using 66924-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 66924-1-Ig (NFATC3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thymus using 66924-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 66924-1-Ig (NFATC3 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells, K-562 cells, Daudi cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66924-1-Ig targets NFATC3 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Affinity:
  K_D=1.16 x 10^-10M
  K_Off=5.67 x 10^-6M
  K_On=4.90 x 10^4M
• Immunogen: NFATC3 fusion protein Ag27306
• Full Name: nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3
• Calculated Molecular Weight: 116 kDa
• Observed Molecular Weight: 160 kDa
• GenBank Accession Number: BC001050
• Gene Symbol: NFATC3
• Gene ID (NCBI): 4775
• RRID: AB_2882251
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NFAT (nuclear factors of activated T cells) proteins are a family of transcription factors originally identified as mediators of activation of cytokine genes in response to antigenic stimulation of T cells. NFAT proteins also play varied roles in cells outside of the immune system [PMID:11877454]. NFATc3 has specifically been implicated in vasculature development, regulation of smooth muscle contractile phenotype, and modulation of vascular smooth muscle contractility [PMID:11439183,17148444]. The calculated molecular weight of NFATC3 is 115 kDa, but the post-modified protein is about 130-170 kDa (PMID: 15728531, PMID: 15857835)