NFATC3 Monoclonal antibody

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  WB analysis using 66924-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66924-1-Ig (NFATC3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human thymus using 66924-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 66924-1-Ig (NFATC3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thymus using 66924-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 66924-1-Ig (NFATC3 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SKOV-3 using 66924-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed SKOV-3 cells using NFATC3 antibody (66924-1-Ig, Clone: 1C1H8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

NFATC3 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 66924-1-Ig

Clone No.1C1H8

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

1C1H8, NF AT4, NF ATc3, NFAT4, NF-AT4

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Tested Applications

• Positive WB detected in: Jurkat cells, K-562 cells, Daudi cells
• Positive IHC detected in: human thymus tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SKOV-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:150-1:600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66924-1-Ig targets NFATC3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Affinity:
  K_D=1.16 x 10^-10M
  K_Off=5.67 x 10^-6M
  K_On=4.90 x 10^4M
• Immunogen:

  CatNo: Ag27306

  Product name: Recombinant human NFATC3 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-205 aa of BC001050

  Sequence: MTTANCGAHDELDFKLVFGEDGAPAPPPPGSRPADLEPDDCASIYIFNVDPPPSTLTTPLCLPHHGLPSHSSVLSPSFQLQSHKNYEGTCEIPESKYSPLGGPKPFECPSIQITSISPNCHQELDAHEDDLQINDPEREFLERPSRDHLYLPLEPSYRESSLSPSPASSISSRSWFSDASSCESLSHIYDDVDSELNEAAARFTL

• Full Name: nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3
• Calculated Molecular Weight: 116 kDa
• Observed Molecular Weight: 160 kDa
• GenBank Accession Number: BC001050
• Gene Symbol: NFATC3
• Gene ID (NCBI): 4775
• RRID: AB_2882251
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q12968
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NFAT (nuclear factors of activated T cells) proteins are a family of transcription factors originally identified as mediators of activation of cytokine genes in response to antigenic stimulation of T cells. NFAT proteins also play varied roles in cells outside of the immune system [PMID:11877454]. NFATc3 has specifically been implicated in vasculature development, regulation of smooth muscle contractile phenotype, and modulation of vascular smooth muscle contractility [PMID:11439183,17148444]. The calculated molecular weight of NFATC3 is 115 kDa, but the post-modified protein is about 130-170 kDa (PMID: 15728531, PMID: 15857835)