NESG1 Polyclonal antibody

NESG1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 21077-1-AP

Synonyms

CCDC19, CFAP45

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Validation Data Gallery

• 

  WB analysis of rat testis using 21077-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 21077-1-AP (NESG1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21077-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21077-1-AP (NESG1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 21077-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 21077-1-AP (NESG1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse testis tissue, rat testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

21077-1-AP targets NESG1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NESG1 fusion protein Ag15257
• Full Name: coiled-coil domain containing 19
• Calculated Molecular Weight: 551 aa, 66 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC027874
• Gene Symbol: CCDC19
• Gene ID (NCBI): 25790
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCDC19, also named as NESG1, is specifically expressed in human nasopharynx and trachea, and decreased or absent in nasopharyngeal carcinoma (NPC) tissues and cell lines. In addition, the levels of NESG1 protein are significantly greater in the low-grade NPC tissues than that in the high-grade NPC tissues. NESG1 also significantly regulats the expression of cell cycle regulators CCNA1 and p21. NESG1 may act as a tumor suppressor by inhibiting cell proliferation, invasion and migration of NPC cells.

Publications

Species	Application	Title
human	WB	Cell Death Dis; Loss of connective tissue growth factor as an unfavorable prognosis factor activates miR-18b by PI3K/AKT/C-Jun and C-Myc and promotes cell growth in nasopharyngeal carcinoma.; Authors - X Yu
human	WB,IHC	J Cell Mol Med; Candidate tumour suppressor CCDC19 regulates miR-184 direct targeting of C-Myc thereby suppressing cell growth in non-small cell lung cancers.; Authors - Zhen Liu ; KD Validated