NEK2 Polyclonal antibody, PBS Only

NEK2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14233-1-PBS

Synonyms

NimA like protein kinase 1, Never in mitosis A-related kinase 2, NEK2A, HsPK21, HSPK 21

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Validation Data Gallery

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  WB analysis using 14233-1-AP (same clone as 14233-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14233-1-AP (NEK2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14233-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 14233-1-AP (same clone as 14233-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 14233-1-AP (NEK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14233-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 14233-1-AP (same clone as 14233-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 14233-1-AP (NEK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14233-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 14233-1-AP (same clone as 14233-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14233-1-AP (NEK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14233-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 14233-1-AP (same clone as 14233-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 14233-1-AP (NEK2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14233-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 14233-1-AP (same clone as 14233-1-PBS)

  Immunofluorescent analysis of Hela cells, using NEK2 antibody 14233-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 14233-1-PBS in a different storage buffer formulation.

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Product Information

14233-1-PBS targets NEK2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NEK2 fusion protein Ag5474
• Full Name: NIMA (never in mitosis gene a)-related kinase 2
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 45-52 kDa
• GenBank Accession Number: BC043502
• Gene Symbol: NEK2
• Gene ID (NCBI): 4751
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.