NECAB2 Polyclonal antibody

NECAB2 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 12257-1-AP

Synonyms

EFCBP2, NECAB2, Stip 2

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Validation Data Gallery

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  IHC staining of mouse brain using 12257-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12257-1-AP (NECAB2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 12257-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12257-1-AP (NECAB2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

12257-1-AP targets NECAB2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NECAB2 fusion protein Ag2909
• Full Name: N-terminal EF-hand calcium binding protein 2
• Calculated Molecular Weight: 386 aa, 42 kDa
• GenBank Accession Number: BC016979
• Gene Symbol: NECAB2
• Gene ID (NCBI): 54550
• RRID: AB_2877841
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IF	Science; miR-183 cluster scales mechanical pain sensitivity by regulating basal and neuropathic pain genes.; Authors - Changgeng Peng
mouse	IF	Development; Termination of cell-type specification gene programs by miR-183 cluster determines the population sizes of low threshold mechanosensitive neurons.; Authors - Changgeng Peng
human	WB	Signal Transduct Target Ther; Multi-omic characterization of genome-wide abnormal DNA methylation reveals diagnostic and prognostic markers for esophageal squamous-cell carcinoma.; Authors - Yiyi Xi
mouse	IHC	Elife; Inhibitory CCK+ basket synapse defects in mouse models of dystroglycanopathy; Authors - Jennifer N Jahncke