NEB-Specific Polyclonal antibody

NEB-Specific Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 19706-1-AP

Synonyms

NEB, NEB177D, nebulin, NEM2

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Validation Data Gallery

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  IHC staining of human skeletal muscle using 19706-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 19706-1-AP (NEB-Specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 19706-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 19706-1-AP (NEB-Specific antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

19706-1-AP targets NEB-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: nebulin
• Calculated Molecular Weight: 773 kDa
• GenBank Accession Number: NM_004543
• Gene Symbol: NEB
• Gene ID (NCBI): 4703
• RRID: AB_10637999
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NEB, a giant muscle protein, may be involved in maintaining the structural integrity of sarcomeres and the membrane system associated with the myofibrils. NEB binds and stabilizes F-actin. Defects in NEB are the cause of nemaline myopathy type 2 (NEM2). The antibody is specific to NEB.

Publications

Species	Application	Title
human	WB	J Clin Invest; KLHL40 deficiency destabilizes thin filament proteins and promotes nemaline myopathy.; Authors - Ankit Garg
mouse	WB	Elife; KLHL41 stabilizes skeletal muscle sarcomeres by nonproteolytic ubiquitination.; Authors - Andres Ramirez-Martinez