Tested Applications
| Positive WB detected in | mouse heart tissue, A549 cells, rat heart tissue |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | human prostate cancer tissue, mouse brain tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:20000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15301-1-AP targets NDUFV2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7559 Product name: Recombinant human NDUFV2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-249 aa of BC001632 Sequence: MFFSAALRARAAGLTAHWGRHVRNLHKTVMQNGAGGALFVHRDTPENNPDTPFDFTPENYKRIEAIVKNYPEGHKAAAVLPVLDLAQRQNGWLPISAMNKVAEVLQVPPMRVYEVATFYTMYNRKPVGKYHIQVCTTTPCMLRNSDSILEAIQKKLGIKVGETTPDKLFTLIEVECLGACVNAPMVQINDNYYEDLTAKDIEEIIDELKAGKIPKPGPRSGRFSCEPAGGLTSLTEPPKGPGFGVQAGL Predict reactive species |
| Full Name | NADH dehydrogenase (ubiquinone) flavoprotein 2, 24kDa |
| Calculated Molecular Weight | 27 kDa |
| Observed Molecular Weight | 24-27 kDa |
| GenBank Accession Number | BC001632 |
| Gene Symbol | NDUFV2 |
| Gene ID (NCBI) | 4729 |
| RRID | AB_2149048 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P19404 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The NDUFV2 gene encodes the 24-kD subunit of the mitochondrial NADH:ubiquinone oxidoreductase (complex I of the respiratory chain). The protein belongs to the complex I 24 kDa subunit family. It is the core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. NDUFV2 constitutes one genetic risk factor for PD, and the mutation may well be a cause of complex I deficiency in this disease(PMID:9570948).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NDUFV2 antibody 15301-1-AP | Download protocol |
| IHC protocol for NDUFV2 antibody 15301-1-AP | Download protocol |
| IP protocol for NDUFV2 antibody 15301-1-AP | Download protocol |
| WB protocol for NDUFV2 antibody 15301-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-NDUFV2 antibody (Cat# 15301-1-AP) has 57 citations published in high-impact journals including Nature, Nat Metab, Nat Cell Biol, Nat Commun, Mol Cell, Cell Reports, Dev Cell, EMBO J, Theranostics, and Advanced Science. Research fields span mitochondrial biology and respiratory chain complex I, cancer metabolism, cardiology, neurology, aging, toxicology, immunology, ophthalmology, and nephrology.
| Species | Application | Title |
|---|---|---|
Nat Metab Sex-specific genetic regulation of adipose mitochondria and metabolic syndrome by Ndufv2. | ||
Nat Cell Biol AIDA directly connects sympathetic innervation to adaptive thermogenesis by UCP1. | ||
Nat Commun Low abundance of the matrix arm of complex I in mitochondria predicts longevity in mice. | ||
