NDUFS8 Polyclonal antibody

NDUFS8 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 25172-1-AP

Synonyms

CI 23kD, Complex I 23kD, NDUFS8, TYKY, TYKY subunit

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Validation Data Gallery

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  WB analysis of mouse brain using 25172-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25172-1-AP (NDUFS8 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 25172-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 25172-1-AP (NDUFS8 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 25172-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25172-1-AP (NDUFS8 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 25172-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25172-1-AP (NDUFS8 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse heart tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 25172-1-AP is NDUFS8 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NDUFS8 fusion protein Ag18531
• Full Name: NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase)
• Calculated Molecular Weight: 210 aa, 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC119754
• Gene Symbol: NDUFS8
• Gene ID (NCBI): 4728
• RRID: AB_2879939
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Biomed Pharmacother; Combination of RNA-seq and proteomics reveals the mechanism of DL0410 treatment in APP/PS1 transgenic mouse model of Alzheimer's disease; Authors - Baoyue Zhang