NDUFB8 Recombinant antibody

NDUFB8 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

230510B10

Cat no : 83216-3-RR

Synonyms

Complex I-ASHI, CI-ASHI, CIASHI, CI ASHI, ASHI

---

Validation Data Gallery

• 

  WB analysis using 83216-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83216-3-RR (NDUFB8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 83216-3-RR

  IP result of anti-NDUFB8 (IP:83216-3-RR, 4ug; Detection:83216-3-RR 1:3000) with HepG2 cells lysate 1470 ug.

• 

  IHC staining of human stomach cancer using 83216-3-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83216-3-RR (NDUFB8 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 83216-3-RR

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83216-3-RR (NDUFB8 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 83216-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFB8 antibody (83216-3-RR, Clone: 230510B10 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 83216-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFB8 antibody (83216-3-RR, Clone: 230510B10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  FC experiment of HeLa using 83216-3-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug NDUFB8 Recombinant antibody (83216-3-RR, Clone:230510B10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 83216-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83216-3-RR against Human NDUFB8 were performed. The affinity constant is 3.26 nM.

---

Tested Applications

• Positive WB detected in: HepG2 cells, A549 cells, mouse liver tissue, mouse brain tissue, rat brain tissue, HeLa cells, Jurkat cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human stomach cancer tissue, human intrahepatic cholangiocarcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83216-3-RR is NDUFB8 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NDUFB8 fusion protein Ag6495
• Full Name: NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 19-22 kDa
• GenBank Accession Number: BC000466
• Gene Symbol: NDUFB8
• Gene ID (NCBI): 4714
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.