NDUFB8 Monoclonal antibody

• Featured Product
• KD/KO Validated

NDUFB8 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse , rat, rabbit, pig and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

7

CloneNo.

3B12C6

Reviews (1)

Cat no : 67690-1-Ig

Synonyms

ASHI, CI ASHI, Complex I ASHI, NDUFB8

---

Validation Data Gallery

• 

  WB analysis using 67690-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67690-1-Ig (NDUFB8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 67690-1-Ig

  WB result of NDUFB8 antibody (67690-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NDUFB8 transfected A549 cells.

• 

  WB analysis using 67690-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67690-1-Ig (NDUFB8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 67690-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67690-1-Ig (NDUFB8 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 67690-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67690-1-Ig (NDUFB8 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 67690-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67690-1-Ig (NDUFB8 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: A549 cells, K-562 cells, HeLa cells, HepG2 cells, Jurkat cells, pig brain tissue, rat brain tissue, mouse brain tissue, rabbit brain tissue
• Positive IHC detected in: human liver tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below

Product Information

67690-1-Ig targets NDUFB8 in WB, IHC, ELISA applications and shows reactivity with Human, mouse , rat, rabbit, pig samples.

• Tested Reactivity: Human, mouse , rat, rabbit, pig
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NDUFB8 fusion protein Ag6569
• Full Name: NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 18-22 kDa
• GenBank Accession Number: BC000466
• Gene Symbol: NDUFB8
• Gene ID (NCBI): 4714
• RRID: AB_2882883
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Front Aging Neurosci; Hippocampus-Based Mitochondrial Respiratory Function Decline Is Responsible for Perioperative Neurocognitive Disorders.; Authors - Keqiang He
mouse	WB	Phytomedicine; Zhen Wu decoction represses renal fibrosis by invigorating tubular NRF2 and TFAM to fuel mitochondrial bioenergetics; Authors - Min Zheng
mouse	WB	Phytother Res; Rutin-activated adipose tissue thermogenesis is correlated with increased intestinal short-chain fatty acid levels.; Authors - Long Cheng
rat	WB	Front Endocrinol (Lausanne); Androgen Excess Induced Mitochondrial Abnormality in Ovarian Granulosa Cells in a Rat Model of Polycystic Ovary Syndrome.; Authors - Linyi Song
rat	WB	Sci Total Environ; Fluoride impairs mitochondrial translation by targeting miR-221-3p/c-Fos/RMND1 axis contributing to neurodevelopment defects; Authors - Dongjie Li
mouse	WB	Chin J Nat Med; Mulberry leaf flavonoids activate BAT and induce browning of WAT to improve type 2 diabetes via regulating the AMPK/SIRT1/PGC-1α signaling pathway; Authors - Long Cheng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Hua (Verified Customer) (06-17-2021)

Good and strong antibody, but with some non-specific bands

• Applications: Western Blot
• Primary Antibody Dilution: 1:8000
• Cell Tissue Type: HepG2, mouse liver and mouse brain

NDUFB8 Antibody Western Blot validation (1:8000 dilution) in HepG2, mouse liver and mouse brain (Cat no:67690-1-Ig)