NDUFB3 Polyclonal antibody, PBS Only

NDUFB3 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12358-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of human heart using 12358-1-AP (same clone as 12358-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 12358-1-AP (NDUFB3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12358-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 12358-1-AP (same clone as 12358-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12358-1-AP (NDUFB3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12358-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat heart using 12358-1-AP (same clone as 12358-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 12358-1-AP (NDUFB3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12358-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 12358-1-AP (same clone as 12358-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12358-1-AP (NDUFB3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12358-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 12358-1-AP (same clone as 12358-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12358-1-AP (NDUFB3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12358-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 12358-1-AP (same clone as 12358-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12358-1-AP (NDUFB3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12358-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 12358-1-AP (same clone as 12358-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12358-1-AP (NDUFB3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12358-1-PBS in a different storage buffer formulation.

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Product Information

12358-1-PBS targets NDUFB3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NDUFB3 fusion protein Ag3022
• Full Name: NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 3, 12kDa
• Calculated Molecular Weight: 98 aa, 11 kDa
• Observed Molecular Weight: 11 kDa
• GenBank Accession Number: BC018183
• Gene Symbol: NDUFB3
• Gene ID (NCBI): 4709
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

NDUFB3(NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3) is also named as CI-B12 and belongs to the complex I NDUFB3 subunit family.It is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis.