NDEL1 Polyclonal antibody

NDEL1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 17262-1-AP

Synonyms

EOPA, MITAP1, Mitosin associated protein 1, NDEL1, NUDEL, Protein Nudel

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Validation Data Gallery

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  WB analysis of human brain using 17262-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17262-1-AP (NDEL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 17262-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17262-1-AP (NDEL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 17262-1-AP

  IP result of anti-NDEL1 (IP:17262-1-AP, 4ug; Detection:17262-1-AP 1:300) with HepG2 cells lysate 2400ug.

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Tested Applications

• Positive WB detected in: human brain tissue, HepG2 cells
• Positive IP detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

17262-1-AP targets NDEL1 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NDEL1 fusion protein Ag10975
• Full Name: nudE nuclear distribution gene E homolog (A. nidulans)-like 1
• Calculated Molecular Weight: 345 aa, 38 kDa, 41 kDa
• Observed Molecular Weight: 38-42 kDa
• GenBank Accession Number: BC026101
• Gene Symbol: NDEL1
• Gene ID (NCBI): 81565
• RRID: AB_2235821
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nuclear distribution factor E-homolog 1 (NDE1), NDE-like 1 (NDEL1), and Lissencephaly 1 (LIS1) together involve in essential neurodevelopmental processes, including neuronal precursor proliferation and differentiation, neurite outgrowth, and neuronal migration. All three bind directly to Disrupted in Schizophrenia 1 (DISC1), a scaffold protein critical to neuronal proliferation, integration, migration, and synaptic function within the developing and adult brain.[PMID: 18469341] Nuclear distribution element-like 1 (NDEL1) was firstly identified as a regulator of the cytoskeleton in microtubule and intermediate fliament dynamics and microtubule-based transport [PMID:21948775]. It is required for organization of the cellular microtubule array and microtubule anchoring at the centrosome, which is in part by targeting the microtubule severing protein KATNA1 to the centrosome[PMID:15728732].

Publications

Species	Application	Title
mouse	WB,IF	J Cell Biol; Lis1 is essential for cortical microtubule organization and desmosome stability in the epidermis.; Authors - Sumigray Kaelyn D KD
mouse	IP, WB	Elife; Sequential phosphorylation of NDEL1 by the DYRK2-GSK3β complex is critical for neuronal morphogenesis.; Authors - Youngsik Woo
human	WB,IF	Sci Rep; Regulation of the actin cytoskeleton by the Ndel1-Tara complex is critical for cell migration.; Authors - Ji-Ho Hong
human	WB	Ann Neurol; Extraciliary roles of the ciliopathy protein JBTS17 in mitosis and neurogenesis.; Authors - Hyowon Hong
mouse	WB	Proc Natl Acad Sci U S A; Gcap14 is a microtubule plus-end-tracking protein coordinating microtubule-actin crosstalk during neurodevelopment; Authors - Dong Jin Mun