NDC80 Polyclonal antibody

NDC80 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 18932-1-AP

Synonyms

HEC, HEC1, HsHec1, hsNDC80, Kinetochore protein Hec1, KNTC2, NDC80, TID3

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Validation Data Gallery

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  WB analysis using 18932-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18932-1-AP (NDC80 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A2780 using 18932-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 18932-1-AP (NDC80 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A2780 using 18932-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 18932-1-AP (NDC80 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 18932-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 18932-1-AP (NDC80 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of A2780 using 18932-1-AP

  IP Result of anti-NDC80 (IP:18932-1-AP, 4ug; Detection:18932-1-AP 1:500) with A2780 cells lysate 960ug.

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  IHC staining of human brain using 18932-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18932-1-AP (NDC80 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 18932-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18932-1-AP (NDC80 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, A2780 cells, human spleen tissue, Jurkat cells, mouse testis tissue
• Positive IP detected in: A2780 cells
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below
• IP: See 1 publications below

Product Information

18932-1-AP targets NDC80 in WB, IP, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.

• Tested Reactivity: human, rat, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NDC80 fusion protein Ag8065
• Full Name: NDC80 homolog, kinetochore complex component (S. cerevisiae)
• Calculated molecular weight: 74 kDa
• Observed molecular weight: 76 kDa
• GenBank accession number: BC035617
• Gene symbol: NDC80
• Gene ID (NCBI): 10403
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IP	J Cell Biol; PP6 regulation of Aurora A-TPX2 limits NDC80 phosphorylation and mitotic spindle size; Authors - Tomoaki Sobajima
human	WB	Oncotarget; Co-expression of mitosis-regulating genes contributes to malignant progression and prognosis in oligodendrogliomas.; Authors - Yanwei Liu
human	WB,IHC	Front Pharmacol; Upregulation of nuclear division cycle 80 contributes to therapeutic resistance via the promotion of autophagy-related protein-7-dependent autophagy in lung cancer; Authors - Xi Chen ; KD Validated
human	WB	Onco Targets Ther; Targeting DTL induces cell cycle arrest and senescence and suppresses cell growth and colony formation through TPX2 inhibition in human hepatocellular carcinoma cells.; Authors - Yu-Chia Chen
	IHC	iScience; NDC80 status pinpoints mitotic kinase inhibitors as emerging therapeutic options in clear cell renal cell carcinoma; Authors - Cheng Hu