NDC80 Polyclonal antibody
NDC80 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat, mouse
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 18932-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, A2780 cells, human spleen tissue, Jurkat cells, mouse testis tissue |
Positive IP detected in | A2780 cells |
Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 3 publications below |
IHC | See 2 publications below |
IP | See 1 publications below |
Product Information
18932-1-AP targets NDC80 in WB, IP, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.
Tested Reactivity | human, rat, mouse |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | NDC80 fusion protein Ag8065 |
Full Name | NDC80 homolog, kinetochore complex component (S. cerevisiae) |
Calculated molecular weight | 74 kDa |
Observed molecular weight | 76 kDa |
GenBank accession number | BC035617 |
Gene symbol | NDC80 |
Gene ID (NCBI) | 10403 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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WB protocol for NDC80 antibody 18932-1-AP | Download protocol |
IHC protocol for NDC80 antibody 18932-1-AP | Download protocol |
IP protocol for NDC80 antibody 18932-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Cell Biol PP6 regulation of Aurora A-TPX2 limits NDC80 phosphorylation and mitotic spindle size | ||
Oncotarget Co-expression of mitosis-regulating genes contributes to malignant progression and prognosis in oligodendrogliomas. | ||
Front Pharmacol Upregulation of nuclear division cycle 80 contributes to therapeutic resistance via the promotion of autophagy-related protein-7-dependent autophagy in lung cancer
| ||
Onco Targets Ther Targeting DTL induces cell cycle arrest and senescence and suppresses cell growth and colony formation through TPX2 inhibition in human hepatocellular carcinoma cells. | ||
iScience NDC80 status pinpoints mitotic kinase inhibitors as emerging therapeutic options in clear cell renal cell carcinoma |