MT-ND5 Polyclonal antibody

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  WB analysis using 55410-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 55410-1-AP (ND5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 55410-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55410-1-AP (MT-ND5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 55410-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55410-1-AP (ND5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 55410-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55410-1-AP (ND5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MT-ND5 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 55410-1-AP

Publications(36)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, ELISA

Synonyms

ND5, ND 5, NADH5, MTND5, MT ND5

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Tested Applications

• Positive WB detected in: mouse liver tissue, rat liver tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 36 publications below

Product Information

55410-1-AP targets MT-ND5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: NADH dehydrogenase, subunit 5 (complex I)
• Calculated Molecular Weight: 67 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: AF465942
• Gene Symbol: MT-ND5
• Gene ID (NCBI): 4540
• RRID: AB_2881324
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P03915
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ND5 belongs to the complex I subunit 1 family. ND5 is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell Metab; Malic enzyme 2 connects the Krebs cycle intermediate fumarate to mitochondrial biogenesis.; Authors - Yi-Ping Wang
human	WB	Mol Cell; Hepatic micropeptide modulates mitochondrial RNA processing machinery in hepatocellular carcinoma; Authors - Linyu Zhu
human	WB	Mol Cell; Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.; Authors - Bin Jiang
human	WB	Sci Adv; MCART1/SLC25A51 is required for mitochondrial NAD transport.; Authors - Nora Kory
human	WB	EMBO J; EglN2 associates with the NRF1-PGC1α complex and controls mitochondrial function in breast cancer.; Authors - Jing Zhang
human	WB	EMBO Rep; MITRAC15/COA1 promotes mitochondrial translation in a ND2 ribosome-nascent chain complex.; Authors - Cong Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Baptiste (Verified Customer) (05-23-2025)

Working well but have a light unspecific (?) band close to ND5.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Lymphoblastoid

MT-ND5 Antibody Western Blot validation (1:500 dilution) in Lymphoblastoid (Cat no:55410-1-AP)