MT-ND5 Polyclonal antibody

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• MT-ND5 Polyclonal antibody (55410-1-AP)

MT-ND5 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 55410-1-AP

Publications(44)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, ELISA

Synonyms

ND5, MT ND5, MTND5, NADH5, ND 5

• 

  WB analysis using 55410-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 55410-1-AP (ND5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 55410-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55410-1-AP (MT-ND5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver using 55410-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55410-1-AP (ND5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver using 55410-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55410-1-AP (ND5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 55410-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55410-1-AP (MT-ND5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse kidney using 55410-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 55410-1-AP (MT-ND5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat kidney using 55410-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 55410-1-AP (MT-ND5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse liver tissue, rat liver tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55410-1-AP targets MT-ND5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: NADH dehydrogenase, subunit 5 (complex I)
• Calculated Molecular Weight: 67 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: AF465942
• Gene Symbol: MT-ND5
• Gene ID (NCBI): 4540
• RRID: AB_2881324
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P03915
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ND5 belongs to the complex I subunit 1 family. ND5 is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	WB	Signal Transduct Target Ther; Fructose directly remodels the translocase of the outer membrane to impair oxidative phosphorylation in podocytes; Authors - Wenyuan Wu
		Cancer Cell; Multiomic integration reveals tumoral heterogeneity of lipid dependence within lethal group 3 medulloblastoma.; Authors - Flavia Bernardi
	WB	Science; Boosting neuronal activity-driven mitochondrial DNA transcription improves cognition in aged mice; Authors - Wenwen Li
human	WB	Cell; Dynamic mapping of proteome trafficking within and between living cells by TransitID; Authors - Wei Qin
human	WB	Cell; PAX translocations remodel mitochondrial metabolism through altered leucine usage in rhabdomyosarcoma; Authors - Bhargab Kalita
human	WB	Cell Metab; Malic enzyme 2 connects the Krebs cycle intermediate fumarate to mitochondrial biogenesis.; Authors - Yi-Ping Wang

Reviews

3.5 out of 5

2 verified customer reviews

What customers say

Automatically generated from the verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Prakash (Verified Customer) (10-28-2025)

its OK in western blot

• Applications: Western Blot
• Cell Tissue Type: Bladder cancer cells

Baptiste (Verified Customer) (05-23-2025)

Working well but have a light unspecific (?) band close to ND5.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Lymphoblastoid

MT-ND5 Antibody Western Blot validation (1:500 dilution) in Lymphoblastoid (Cat no:55410-1-AP)