NCLN Polyclonal antibody

NCLN Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10985-1-AP

Synonyms

NCLN, Nicalin, nicalin homolog (zebrafish), Nicastrin like protein

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Validation Data Gallery

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  WB analysis of mouse pancreas using 10985-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 10985-1-AP (NCLN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach cancer using 10985-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10985-1-AP (NCLN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 10985-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10985-1-AP (NCLN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 10985-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10985-1-AP (NCLN antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 10985-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10985-1-AP (NCLN antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 10985-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10985-1-AP (NCLN antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 10985-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10985-1-AP (NCLN antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse pancreas tissue
• Positive IHC detected in: human stomach cancer tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10985-1-AP targets NCLN in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NCLN fusion protein Ag1430
• Full Name: nicalin homolog (zebrafish)
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 60-64 kDa
• GenBank Accession Number: BC013283
• Gene Symbol: NCLN
• Gene ID (NCBI): 56926
• RRID: AB_2150898
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nicalin, also named as NCLN, is a key component of a protein complex that antagonizes Nodal signaling, which in vertebrates is involved in induction of the mesoderm and endoderm. Nicalin is highest expression in pancreas and skeletal muscle, with somewhat lower expression in heart and very low expression in all other tissues examined. Endogenous nicalin has an apparent molecular mass of 60 kDa. Nicalin was also detected in a 500- to 550-kDa complex containing NOMO2.