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  • KD/KO Validated

NCAPG Polyclonal antibody

NCAPG Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 24563-1-AP

Synonyms

CAPG, CHCG, Condensin complex subunit 3, Condensin subunit CAP G, HCAP G, Melanoma antigen NY MEL 3, NCAPG, NY MEL 3, NYMEL3, XCAP G homolog



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman testis tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 24563-1-AP is NCAPG Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NCAPG fusion protein Ag19926
Full Name non-SMC condensin I complex, subunit G
Calculated molecular weight 1015 aa, 114 kDa
Observed molecular weight131 kDa
GenBank accession numberBC068467
Gene symbol NCAPG
Gene ID (NCBI) 64151
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NCAPG antibody 24563-1-APDownload protocol
IHC protocol for NCAPG antibody 24563-1-APDownload protocol
IF protocol for NCAPG antibody 24563-1-APDownload protocol
IP protocol for NCAPG antibody 24563-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Oncol

NCAPG Promotes Tumor Progression and Modulates Immune Cell Infiltration in Glioma.

Authors - Guangrong Zheng
  • KD Validated
human,mouseWB,IHC,CoIP

J Transl Med

NCAPG promotes the proliferation of hepatocellular carcinoma through the CKII-dependent regulation of PTEN.

Authors - Rongguiyi Zhang
  • KD Validated
humanWB

J Gastrointest Oncol

NCAPG is transcriptionally regulated by CBX3 and activates the Wnt/β-catenin signaling pathway to promote proliferation and the cell cycle and inhibit apoptosis in colorectal cancer

Authors - Hong Yang
  • KD Validated
humanWB,IHC

Cell Death Dis

NCAPG confers trastuzumab resistance via activating SRC/STAT3 signaling pathway in HER2-positive breast cancer.

Authors - Lili Jiang
  • KD Validated
humanIHC

Sci Rep

Adipose-derived mesenchymal stem cells differentiate into heterogeneous cancer-associated fibroblasts in a stroma-rich xenograft model.

Authors - Yoshihiro Miyazaki
humanWB,IHC

Front Pharmacol

Identification and verification of hub genes associated with the progression of non-small cell lung cancer by integrated analysis

Authors - Xie Mengyan