NBN / NBS1 Polyclonal antibody

• Featured Product
• KD/KO Validated

NBN / NBS1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 55025-1-AP

Synonyms

NBN, hNbs1, FLJ10155, ATV, AT V2

---

Validation Data Gallery

• 

  WB analysis of HeLa using 55025-1-AP

  WB result of NBS1 antibody (55025-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NBS1 transfected HeLa cells.

• 

  WB analysis of HeLa using 55025-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55025-1-AP (NBS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 55025-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 55025-1-AP (NBS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 55025-1-AP

  IP result of anti-NBN / NBS1 (IP:55025-1-AP, 4ug; Detection:55025-1-AP 1:3000) with HeLa cells lysate 1320 ug.

• 

  IHC staining of human stomach using 55025-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 55025-1-AP (NBN / NBS1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 55025-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 55025-1-AP (NBN / NBS1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 55025-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using NBN / NBS1 antibody (55025-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of A549 using 55025-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using 55025-1-AP (NBS1 antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: HeLa cells, human testis tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 12 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

55025-1-AP targets NBN / NBS1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: nibrin
• Calculated Molecular Weight: 85 kDa
• Observed Molecular Weight: 90-95 kDa
• GenBank Accession Number: NM_002485
• Gene Symbol: NBN
• Gene ID (NCBI): 4683
• RRID: AB_10858928
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NBN, also named as NBS, NBS1 and P95, is a component of the MRE11/RAD50/NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBN is involved in G1 and G2 checkpoints. Defects in NBN are the cause of Nijmegen breakage syndrome (NBS). Defects in NBN are a cause of genetic susceptibility to breast cancer (BC). Defects in NBN may be associated with aplastic anemia. Defects in NBN might play a role in the pathogenesis of childhood acute lymphoblastic leukemia (ALL). The antibody is specific to NBN. The full-length NBN protein, with an apparent molecular weight of 95 kDa and the two protein fragments of 26 and 70 kDa arising from the c.657_661del5 (p.K219fsX19) mutation, and the 80 kDa protein found in patient RR with the mutation c.742_743insGG leading to excision of exons 6 and 7 from the NBN mRNA are shown. (PMID: 26265251) The predicted molecular weight of NBN protein (p95) is 85kDa, actually detection result is about 95kDa(PMID: 23762398).

Publications

Species	Application	Title
human	WB	Mol Cell; C1QBP Promotes Homologous Recombination by Stabilizing MRE11 and Controlling the Assembly and Activation of MRE11/RAD50/NBS1 Complex.; Authors - Yongtai Bai
human	WB	Nat Commun; Crosstalk between SUMOylation and ubiquitylation controls DNA end resection by maintaining MRE11 homeostasis on chromatin; Authors - Tao Zhang
human	WB	Nucleic Acids Res; LncRNA lnc-RI regulates homologous recombination repair of DNA double-strand breaks by stabilizing RAD51 mRNA as a competitive endogenous RNA.; Authors - Liping Shen
mouse	WB,IF	Cell Death Differ; NBS1 is required for SPO11-linked DNA double-strand break repair in male meiosis.; Authors - Bin Zhang ; KD Validated
human	WB	Oncogene; TRIM24 is critical for the cellular response to DNA double-strand breaks through regulating the recruitment of MRN complex; Authors - Ya Wang
rat	WB	Mol Cancer Res; PKCð regulates chromatin remodeling and DNA repair through SIRT6; Authors - Trisiani Affandi