CoraLite® Plus 750-conjugated NAGS Recombinant antibody

NAGS Uni-rAb^TM Recombinant Antibody for WB, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra)

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

230279D3

Cat no : CL750-82963

Synonyms

N-acetylglutamate synthase conserved domain form, EC:2.3.1.1, ARGA, Amino-acid acetyltransferase, AGAS

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Validation Data Gallery

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  WB analysis using CL750-82963

  Various lysates were subjected to SDS PAGE followed by western blot with CL750-82963 (NAGS antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  FC experiment of HepG2 using CL750-82963

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 750-conjugated NAGS Recombinant antibody (CL750-82963, Clone:230279D3)(red), or 0.4 ug Isotype Control (CL750-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: rat liver tissue, mouse liver
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL750-82963 targets NAGS in WB, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NAGS fusion protein Ag16073
• Full Name: N-acetylglutamate synthase
• Calculated Molecular Weight: 534 aa, 58 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC111713
• Gene Symbol: NAGS
• Gene ID (NCBI): 162417
• Conjugate: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 755 nm / 780 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

N-acetylglutamate synthase, also known as NAGS, is a mitochondrial enzyme that catalyzes the formation of N-acetylglutamate (NAG) from glutamate and acetyl coenzyme-A. NAGS is the key enzyme for regulating the hepatic urea cycle and is also highly expressed in the kidney and gut. Deficiency of NAGS leads to hyperammonemia due to decreased activity of CPSI deprived of its cofactor NAG(PMID:12459178,12754705).