NAGS Recombinant antibody

NAGS Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230279D3

Cat no : 82963-1-RR

Synonyms

N-acetylglutamate synthase conserved domain form, EC:2.3.1.1, ARGA, Amino-acid acetyltransferase, AGAS

---

Validation Data Gallery

• 

  WB analysis using 82963-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82963-1-RR (NAGS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 82963-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82963-1-RR against Human NAGS were performed. The affinity constant is below 1 pM.

---

Tested Applications

• Positive WB detected in: HepG2 cells, mouse liver tissue, rat liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82963-1-RR targets NAGS in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NAGS fusion protein Ag16073
• Full Name: N-acetylglutamate synthase
• Calculated Molecular Weight: 534 aa, 58 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC111713
• Gene Symbol: NAGS
• Gene ID (NCBI): 162417
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

N-acetylglutamate synthase, also known as NAGS, is a mitochondrial enzyme that catalyzes the formation of N-acetylglutamate (NAG) from glutamate and acetyl coenzyme-A. NAGS is the key enzyme for regulating the hepatic urea cycle and is also highly expressed in the kidney and gut. Deficiency of NAGS leads to hyperammonemia due to decreased activity of CPSI deprived of its cofactor NAG(PMID:12459178,12754705).