Anti-Human N-cadherin/CD325 Rabbit Recombinant Antibody, PBS Only

N-cadherin/CD325 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

230259D7

Cat no : 98006-1-PBS

Synonyms

N-cadherin, CDHN, CDH2, CD325, Cadherin-2

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Validation Data Gallery

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  FC experiment of HeLa using 98006-1-RR (same clone as 98006-1-PBS)

  1x10^6 HeLa cells (left) or MCF-7 cells (right) were surface stained with 0.25 ug Anti-Human N-cadherin/CD325 Rabbit Recombinant Antibody (98006-1-RR, Clone: 230259D7) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Isotype Control (blue). Cells were not fixed. This data was developed using the same antibody clone with 98006-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98006-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98006-1-RR against Human N-cadherin were performed. The affinity constant is 2.73 nM.

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Product Information

98006-1-PBS targets N-cadherin/CD325 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: cadherin 2, type 1, N-cadherin (neuronal)
• Calculated Molecular Weight: 100 kDa
• GenBank Accession Number: NM_001792.4
• Gene Symbol: N-cadherin
• Gene ID (NCBI): 1000
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C. Stable for one year after shipment.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also include E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619).