Myocd Polyclonal antibody

Myocd Polyclonal Antibody for IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 12457-1-AP

Synonyms

BSAC2A, myocardin, Myocd, Srfcp

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Validation Data Gallery

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  IP experiment of HepG2 using 12457-1-AP

  IP result of anti-Myocd (IP:12457-1-AP, 4ug; Detection:12457-1-AP 1:500) with HepG2 cells lysate 1360 ug.

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  IHC staining of human colon cancer using 12457-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12457-1-AP (Myocd antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IP: See 1 publications below

Product Information

12457-1-AP targets Myocd in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Myocd fusion protein Ag3123
• Full Name: myocardin
• Calculated Molecular Weight: 938 aa, 102 kDa
• Observed Molecular Weight: 102kDa, 140kDa
• GenBank Accession Number: NM_001146312
• Gene Symbol: MYOCD
• Gene ID (NCBI): 93649
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB, IP	Biochem Biophys Res Commun; PMID: 25301554; Authors - Jing-Ting Li