MSI2 Polyclonal antibody

MSI2 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 10770-1-AP

Synonyms

Musashi-2, RNA-binding protein Musashi homolog 2, Musashi 2, MSI2H

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Validation Data Gallery

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  WB analysis of PC-12 using 10770-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-12 using 10770-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10770-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 10770-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10770-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 10770-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 10770-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 10770-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10770-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 10770-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody) at dilution of 1:500.

• 

  IHC staining of human ovary tumor using 10770-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10770-1-AP (MSI2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 10770-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10770-1-AP (MSI2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 10770-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10770-1-AP (MSI2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 10770-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10770-1-AP (MSI2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10770-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10770-1-AP (MSI2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10770-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10770-1-AP (MSI2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: PC-12 cells, C6 cells, COLO 320 cells, MCF-7 cells
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human ovary tumor tissue, human colon cancer tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• IP: See 2 publications below

Product Information

10770-1-AP targets MSI2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MSI2 fusion protein Ag1213
• Full Name: musashi homolog 2 (Drosophila)
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 37-40 kDa
• GenBank Accession Number: BC001526
• Gene Symbol: MSI2
• Gene ID (NCBI): 124540
• RRID: AB_2147935
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MSI2 is an RNA-binding protein that regulates the expression of target mRNAs at the translation level. It binds to RNA via 2 ribonucleoprotein-type RNA recognition motifs in their N-termini, to consensus motifs in mRNAs to inhibit transcription. It may play a role in the proliferation and maintenance of stem cells in the central nervous system. MSI2 can regulate normal hematopoiesis and promote aggressive myeloid leukemia

Publications

Species	Application	Title
human	WB,IF	Science; Neurodevelopmental protein Musashi-1 interacts with the Zika genome and promotes viral replication.; Authors - Pavithra L Chavali
human	WB	EMBO J; Suppression of miR-199a maturation by HuR is crucial for hypoxia-induced glycolytic switch in hepatocellular carcinoma.; Authors - Ling-Fei Zhang
human	WB	Int J Oncol; Effects of three-dimensional collagen scaffolds on the expression profiles and biological functions of glioma cells.; Authors - Wei Jia
human	IHC	Mod Pathol; Progenitor stem cell marker expression by pulmonary carcinomas.; Authors - Moreira Andre L AL
human	WB, IP, IF	Front Mol Biosci; RNA-Binding Protein MSI2 Binds to miR-301a-3p and Facilitates Its Distribution in Mitochondria of Endothelial Cells.; Authors - Qian Qian Guo
human	IHC	Exp Cell Res; Msi2 plays a carcinogenic role in esophageal squamous cell carcinoma via regulation of the Wnt/β-catenin and Hedgehog signaling pathways.; Authors - Zhiwei Li