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MultiPro® Human Discovery Panel

The MultiPro® Human Discovery Panel contains 347 DNA-barcoded antibodies used to profile up to 326 protein targets. The Panel contains 5 isotype control antibodies. Designed and validated across diverse sample types and conditions with the 10x Genomics Single Cell Gene Expression Flex with Feature Barcode chemistry.

Host / Isotype

Rabbit polyclonal IgG, Mouse IgG2b, Mouse IgG3, Mouse IgG2a, Mouse IgG1

Reactivity

Human

Cat No : G900150

Validation Data Gallery

  • Lewis x antigen involved in cell-cell adhesion, mediates phagocytosis and chemotaxis. UMAP shows resting Bone Marrow Mononuclear cells

    Lewis x antigen involved in cell-cell adhesion, mediates phagocytosis and chemotaxis. UMAP shows resting Bone Marrow Mononuclear cells

  • E-cadherin, mediates cell-cell adhesion in epithelial tissues. UMAP shows resting Bone Marrow Mononuclear cells

    E-cadherin, mediates cell-cell adhesion in epithelial tissues. UMAP shows resting Bone Marrow Mononuclear cells

  • Stem cell factor receptor, plays essential roles in hematopoiesis, spermatogenesis, and melanogenesis. UMAP shows resting Bone Marrow Mononuclear cells

    Stem cell factor receptor, plays essential roles in hematopoiesis, spermatogenesis, and melanogenesis. UMAP shows resting Bone Marrow Mononuclear cells

  • Protein kinase C and casein kinase substrate in neurons protein 1, involved in endocytosis and actin dynamics. UMAP shows human PBMCs stimulated with anti-CD3/CD28 for 4h

    Protein kinase C and casein kinase substrate in neurons protein 1, involved in endocytosis and actin dynamics. UMAP shows human PBMCs stimulated with anti-CD3/CD28 for 4h

  • H2A histone family member X, involved in DNA repair and chromatin structure. UMAP shows human PBMCs stimulated with anti-CD3/CD28 for 4h

    H2A histone family member X, involved in DNA repair and chromatin structure. UMAP shows human PBMCs stimulated with anti-CD3/CD28 for 4h

  • Transcription factor critical for hematopoietic differentiation and lymphatic system development. UMAP shows resting Bone Marrow Mononuclear cells and in human PBMCs stimulated with LPS for 4h

    Transcription factor critical for hematopoietic differentiation and lymphatic system development. UMAP shows resting Bone Marrow Mononuclear cells and in human PBMCs stimulated with LPS for 4h

  • Drebrin 1, involved in dendritic spine morphogenesis and synaptic plasticity. UMAP shows human PBMCs stimulated with LPS for 4h

    Drebrin 1, involved in dendritic spine morphogenesis and synaptic plasticity. UMAP shows human PBMCs stimulated with LPS for 4h

  • Transcription factor critical for hematopoietic differentiation and lymphatic system development. UMAP shows resting Bone Marrow Mononuclear cells and in human PBMCs stimulated with LPS for 4h

    Transcription factor critical for hematopoietic differentiation and lymphatic system development. UMAP shows resting Bone Marrow Mononuclear cells and in human PBMCs stimulated with LPS for 4h

  • Stem cell factor receptor, plays essential roles in hematopoiesis, spermatogenesis, and melanogenesis. UMAP shows resting Bone Marrow Mononuclear cells

    Stem cell factor receptor, plays essential roles in hematopoiesis, spermatogenesis, and melanogenesis. UMAP shows resting Bone Marrow Mononuclear cells

  • N-cadherin, involved in cell-cell adhesion and signal transduction, crucial in the nervous system and heart. UMAP shows resting PBMCs.

    N-cadherin, involved in cell-cell adhesion and signal transduction, crucial in the nervous system and heart. UMAP shows resting PBMCs.

  • Phosphorylated MAP kinase kinase 1 at Serine 298, part of the MAPK/ERK pathway, involved in cell growth signaling. UMAP shows resting PBMCs.

    Phosphorylated MAP kinase kinase 1 at Serine 298, part of the MAPK/ERK pathway, involved in cell growth signaling. UMAP shows resting PBMCs.

  • Transforming growth factor beta 1, regulates cell growth, proliferation, differentiation, and apoptosis. UMAP shows resting PBMCs.

    Transforming growth factor beta 1, regulates cell growth, proliferation, differentiation, and apoptosis. UMAP shows resting PBMCs.

Download full data set

Product Information

Tested Reactivity Human
Host / Isotype Rabbit polyclonal IgG, Mouse IgG2b, Mouse IgG3, Mouse IgG2a, Mouse IgG1, Mouse IgG2ak, IgG2b, IgMk
Class Monoclonal, Recombinant, and Polyclonal
Type DNA-barcoded Antibody Cocktail
Storage Buffer Lyophilized antibody cocktail
Storage ConditionsStore at 2-8°C.

Targets

Download MultiPro Discovery Antibody Manifest G9000150.xlsm

*This file contains macros that are used to make searching the list of antibodies easier. Make sure to enable macros when prompted. You may also need to “unblock” the file in the file’s properties box.

Protocols

Download Protocol

*We have validated the MultiPro® Human Discovery Panel with the following protocol from 10x Genomics with one important change. The use of Enhanced Blocking Reagent (G900005) is highly recommended for improved signal-to-noise. See protocol for more details.

Sequencing Parameters

IMPORTANT: The sequencing parameters for MultiPro® are distinct from those required for 10x Genomics Gene Expression Flex sequencing libraries. MultiPro™ sequencing parameters are also distinct from other commercially available oligonucleotide conjugated antibodies. Please be mindful of this when setting up your sequencing run. The required MultiPro™ sequencing parameters are:
If using Cell Ranger v8.0+
Read 1: 28 cycles
Index 1:10 cycles
Index 2:10 cycles
Read 2:90 cycles

If using Cell Ranger v7.2
Read 1: 48 cycles
Index 1:10 cycles
Index 2:10 cycles
Read 2: 50 cycles