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MultiProTM 5CFLX Anti-Human CD14 (UCHM-1)

CD14 Monoclonal Antibody for Single Cell

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

Single Cell

Conjugate

5CFLX

CloneNo.

UCHM-1

Cat no : G65056-1-5C

Synonyms

CD14, CD14 molecule

Validation Data Gallery

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  • Single Cell Sequencing experiment G65056-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65056-1-5C on Resting PBMC

    G65056-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

  • Single Cell Sequencing experiment G65056-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65056-1-5C on PBMC treated with 4hr LPS + TI

    G65056-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

Tested Applications

Positive Single Cell detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.

Recommended dilution

ApplicationDilution
SINGLE CELL<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

G65056-1-5C targets CD14 in Single Cell applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Oligo Conjugate
Type Monoclonal
Immunogen Thymocytes and peripheral blood lymphocytes from a remission AML patient
Full Name MultiProTM 5CFLX Anti-Human CD14 (UCHM-1)
Calculated Molecular Weight 375 aa, 40 kDa
GenBank Accession NumberBC010507
Gene Symbol CD14
Gene ID (NCBI) 929
Conjugate 5CFLX
Form Liquid
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C

Background Information

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.

Protocols

MultiPro™ Cell Surface and Intracellular Staining ProtocolDownload protocol
10x Genomics Cell Surface Protein Only Staining ProtocolDownload protocol