Mouse CD45 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: mouse

Detection: mouse

Applications

Sandwich ELISA

Range

Sandwich ELISA: 7.8-500 pg/mL

Cat No : MP00412-4

Print datasheet

Synonyms

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP00412-4

  Sandwich ELISA standard curve of MP00412-4, Mouse CD45 Recombinant Matched Antibody Pair - PBS only. 83396-14-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0845. 83396-7-PBS was HRP conjugated as the detection antibody. Range: 7.8-500 pg/mL.

• 

  Sample test of MP00412-4

  RAW 264.7 were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for mouse CD45, and measured 154.9 pg/mL.

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  Sample test of MP00412-4

  The mean mouse CD45 concentration was determined to be 39.3 ng/mL in RAW 264.7 cell extract based on a 4.8 mg/mL extract load.

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Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP00412-4 targets CD45 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 7.8-500 pg/mL (Sandwich ELISA);

Capture antibody: Mouse CD45 Recombinant antibody, PBS Only (Capture) 83396-14-PBS (240920B4). 100 μg. Concentration 1 mgl/ml.

Detection antibody: Mouse CD45 Recombinant antibody, PBS Only (Detector) 83396-7-PBS (240920A8). 100 μg. Concentration 1 mgl/ml.

Alternative CD45 matched antibody pairs: MP00412-1, MP00412-2, MP00412-3, MP01280-1, MP01280-2, MP50221-1, MP50221-2, MP50221-3, MP50221-4

Matched pair protein standard: Eg0845

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

• Capture antibody
• Detection antibody

• Catalog Number: 83396-14-PBS
• Tested Reactivity: mouse
• Immunogen: Fusion Protein
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Full Name: protein tyrosine phosphatase, receptor type, C
• Synonyms: Ptprc, Leukocyte common antigen, LCA, B220, 240920B4
• GenBank accession number: NM_001111316.2
• Gene symbol: CD45
• Uniprot: P06800-4
• Gene ID (NCBI): 19264
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

• Catalog Number: 83396-7-PBS
• Tested Reactivity: mouse
• Immunogen: Fusion Protein
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Full Name: protein tyrosine phosphatase, receptor type, C
• Synonyms: Ptprc, Leukocyte common antigen, LCA, B220, 240920A8
• GenBank accession number: NM_001111316.2
• Gene symbol: CD45
• Uniprot: P06800-4
• Gene ID (NCBI): 19264
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.