Mouse CD163 ELISA Kit
Cat no : KE10115
Synonyms
Cd163,M130,sCD163
Validation Data Gallery
Product Information
KE10115 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse CD163 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse CD163. The assay recognizes mouse CD163. An antibody specific for mouse CD163 has been pre-coated onto the microwells. The mouse CD163 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse CD163 is added to detect the captured mouse CD163 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse CD163 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 0.9 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 93671 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 108% | 96%-129% |
| Cell culture supernatant | 94% | 78%-118% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 2,581.2 | 168.3 | 6.5 |
| 2 | 20 | 594.0 | 15.1 | 2.5 |
| 3 | 20 | 148.7 | 12.1 | 8.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 2,603.0 | 251.7 | 9.7 |
| 2 | 24 | 606.4 | 59.8 | 9.9 |
| 3 | 24 | 141.9 | 13.9 | 9.8 |
Background Information
CD163, also known as M130, is a membrane glycoprotein which belongs to the scavenger receptor superfamily. It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages. CD163 mediates endocytosis of haptoglobin-haemoglobin complexes. The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response. Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Cd163,M130,sCD163 |