Acetyl-Histone H3 (Lys9) Polyclonal antibody

• Featured Product
• KD/KO Validated

Acetyl-Histone H3 (Lys9) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 29133-1-AP

Synonyms

HIST1H3A, Histone-H3, HistoneH3, Histone H3, Histone H 3

---

Validation Data Gallery

• 

  WB analysis using 29133-1-AP

  Untreated and Trichostatin A treated HeLa cell lysates were subjected to SDS PAGE followed by western blot with 29133-1-AP (Mono-Acetyl-Histone H3 (Lys9) antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 29133-1-AP

  WB result of Mono-Acetyl-Histone H3 (Lys9) antibody (29133-1-AP; 1:2400; incubated at room temperature for 1.5 hours) with sh-Control and sh-Mono-Acetyl-Histone H3 (Lys9) transfected HEK-293 cells.

---

Tested Applications

• Positive WB detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

29133-1-AP targets Acetyl-Histone H3 (Lys9) in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: histone cluster 1, H3a
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC066245
• Gene Symbol: HIST1H3A
• Gene ID (NCBI): 8350
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Histones, including H1/H5 (linker histones), H2, H3, and H4 (core histones), are nucleic proteins which interact with DNA to form the nucleosomes and play important roles in gene regulation and DNA replication. Histone proteins are highly post-translationally modified while Histone H3 is the most extensively modified.

Publications

Species	Application	Title
rat	IHC	Neurochem Res; The Role of Acetylation and Methylation of Rat Hippocampal Histone H3 in the Mechanism of Aluminum-Induced Neurotoxicity; Authors - Jie Gao