Acetyl-Histone H3 (Lys9) Recombinant monoclonal antibody

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• Acetyl-Histone H3 (Lys9) Recombinant monoclonal antibody (84543-1-RR)

Acetyl-Histone H3 (Lys9) Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Dot Blot, ELISA, ChIP-qPCR

Cat No. 84543-1-RR Copied!

Clone No. 241596F9

Publications (12)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, Dot Blot, ELISA, ChIP-qPCR

Synonyms

Acetyl Histone, H3, H3 Ac Lys9, H3 Acetyl K9, H3 Acetyl Lys9

• 

  WB analysis of HEK-293 using 84543-1-RR

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 84543-1-RR (Acetyl-Histone H3 (Lys9) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 84543-1-RR

  Trichostatin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 84543-1-RR (Acetyl-Histone H3 (Lys9) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Alpha Tubulin Monoclonal antibody (66031-1-Ig) as loading control.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 84543-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HIST1H3A antibody (84543-1-RR, Clone: 241596F9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Dot Blot experiment of peptide using 84543-1-RR

  Dot blot analysis was used to confirm the specificity of Acetyl-Histone H3 (Lys9) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  ChIP experiment of HeLa using 84543-1-RR

  Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Acetyl-Histone H3 (Lys9) (84543-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: Trichostatin A treated HeLa cells, HEK-293 cells
• Positive IF/ICC detected in: HeLa cells
• Positive Dot Blot detected in: peptide
• Positive ChIP-qPCR detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• DOT BLOT: DOT BLOT : 1:10-1:100
• CHIP-QPCR: CHIP-QPCR : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84543-1-RR targets Acetyl-Histone H3 (Lys9) in WB, IHC, IF/ICC, Dot Blot, ELISA, ChIP-qPCR applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: histone cluster 1, H3a
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC066245
• Gene Symbol: HIST1H3A
• Gene ID (NCBI): 8350
• RRID: AB_3672048
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P68431
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The Acetyl-Histone H3 (Lys9) recombinant monoclonal antibody (clone 241596F9), catalog number 84543-1-RR, has 12 citations. These appear in journals including Advanced Science, Journal of Translational Medicine, International Journal of Biological Macromolecules, Cell Molecular Gastroenterology and Hepatology, International Immunopharmacology, Chemical & Biological Interactions, Toxicology, Archives of Gerontology and Geriatrics, Cell Signaling, BMC Cancer, and Bone. Research fields span epigenetics, histone acetylation, kidney disease, cancer, cardiovascular pathology, osteoporosis, pulmonary fibrosis, and metabolic disorders.

Automatically generated from the 12 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Adv Sci (Weinh); Miro1 Protects Against Acute Kidney Injury Through Modulating Mitochondrial Homeostasis via Interaction With Sirt6.; Authors - Lin Wu
human	ChIP	J Transl Med; USP22 suppresses trophoblast cell necroptosis in preeclampsia by stabilizing KAT2A-mediated histone acetylation at the SFRP1 promoter.; Authors - Lidan He
human	ChIP	Int J Biol Macromol; Bone marrow tyrosine kinase on chromosome X promotes epithelial-mesenchymal transition through signal transducer and activator of transcription 3 in colorectal cancer.; Authors - Man Zhang
human	WB	Cell Mol Gastroenterol Hepatol; Gut Microbiota-Derived Propionate Governs Hepatic N2 Neutrophils in Wilson's Disease.; Authors - Xiaoxiao Mi
human	ChIP	Int Immunopharmacol; DLX2 drives vascular calcification in chronic kidney disease through glycolytic activation: a mechanism epigenetically regulated by HDAC2.; Authors - Qun Huang
		Chem Biol Interact; A novel histone deacetylase inhibitor suppresses lipid accumulation and ameliorates atherosclerosis in apolipoprotein E-deficient mice.; Authors - Shasha Hu