Marcks Polyclonal antibody

• Featured Product
• KD/KO Validated

Marcks Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse and More (2)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 10004-2-Ig

Synonyms

Macs, Marcks, PKCSL

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Validation Data Gallery

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  WB analysis of mouse brain using 10004-2-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10004-2-Ig (Marcks antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10004-2-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10004-2-Ig (Marcks antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse pancreas using 10004-2-Ig

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10004-2-Ig (Marcks antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 10004-2-Ig

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10004-2-Ig (Marcks antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 10004-2-Ig

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10004-2-Ig (Marcks Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse pancreas using 10004-2-Ig

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10004-2-Ig (Marcks Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IHC detected in: mouse pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Product Information

10004-2-Ig targets Marcks in WB, IHC, IF, IP, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Cited Reactivity: mouse, rat, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: myristoylated alanine rich protein kinase C substrate
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 88 kDa
• GenBank Accession Number: BC046601
• Gene Symbol: Marcks
• Gene ID (NCBI): 17118
• RRID: AB_2140311
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MARCKS is a member of MARCKS family of PKC substrate and it is also a widely accepted indicator of PKC activation. It shows a ubiquitous tissue distribution. The calculated molecular weight of MARCKS is 39 kDa and its apparent molecular weights were found at 68 kDa to 90 kDa in different species. This protein is a known to regulated cytoskeleton remodeling and membrane recycling. The anti-MARCKS antibody is raised against purified MARCKS protein from mouse brain using a method similar to that previously reported for MacMARCKS

Publications

Species	Application	Title
mouse	IF	Dev Cell; An integrated approach identifies the molecular underpinnings of murine anterior visceral endoderm migration; Authors - Shifaan Thowfeequ
bovine	WB	Proc Natl Acad Sci U S A; MARCKS protein mediates hydrogen peroxide regulation of endothelial permeability.; Authors - Jin Benjamin Y BY
mouse	IF	Curr Biol; Stretch triggers microtubule stabilization and MARCKS-dependent membrane incorporation in the shaft of embryonic axons; Authors - Sara C Sousa
mouse	IF	J Cell Sci; Dynamic adhesions and MARCKS in melanoma cells.; Authors - Estrada-Bernal Adriana A
	WB,IP	Carcinogenesis; GRB-7 facilitates HER-2/Neu-mediated signal transduction and tumor formation.; Authors - Bai Tao T
	WB,IHC	J Biol Chem; The long noncoding RNA lncZic2 drives the self-renewal of liver tumor-initiating cells via the protein kinase C substrates MARCKS and MARCKSL1.; Authors - Zhenzhen Chen ; KO Validated