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  • KD/KO Validated

Marcks Polyclonal antibody

Marcks Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse and More (2)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Cat no : 10004-2-Ig

Synonyms

Macs, Marcks, PKCSL



Tested Applications

Positive WB detected inmouse brain tissue
Positive IHC detected inmouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10004-2-Ig targets Marcks in WB, IHC, IF, IP, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Cited Reactivitymouse, rat, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant Protein
Full Name myristoylated alanine rich protein kinase C substrate
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 88 kDa
GenBank Accession NumberBC046601
Gene Symbol Marcks
Gene ID (NCBI) 17118
RRIDAB_2140311
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MARCKS is a member of MARCKS family of PKC substrate and it is also a widely accepted indicator of PKC activation. It shows a ubiquitous tissue distribution. The calculated molecular weight of MARCKS is 39 kDa and its apparent molecular weights were found at 68 kDa to 90 kDa in different species. This protein is a known to regulated cytoskeleton remodeling and membrane recycling. The anti-MARCKS antibody is raised against purified MARCKS protein from mouse brain using a method similar to that previously reported for MacMARCKS

Protocols

Product Specific Protocols
WB protocol for Marcks antibody 10004-2-IgDownload protocol
IHC protocol for Marcks antibody 10004-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Dev Cell

An integrated approach identifies the molecular underpinnings of murine anterior visceral endoderm migration

Authors - Shifaan Thowfeequ
bovineWB

Proc Natl Acad Sci U S A

MARCKS protein mediates hydrogen peroxide regulation of endothelial permeability.

Authors - Jin Benjamin Y BY
mouseIF

Curr Biol

Stretch triggers microtubule stabilization and MARCKS-dependent membrane incorporation in the shaft of embryonic axons

Authors - Sara C Sousa
mouseIF

J Cell Sci

Dynamic adhesions and MARCKS in melanoma cells.

Authors - Estrada-Bernal Adriana A
WB,IP

Carcinogenesis

GRB-7 facilitates HER-2/Neu-mediated signal transduction and tumor formation.

Authors - Bai Tao T
WB,IHC

J Biol Chem

The long noncoding RNA lncZic2 drives the self-renewal of liver tumor-initiating cells via the protein kinase C substrates MARCKS and MARCKSL1.

Authors - Zhenzhen Chen
  • KO Validated