MYO18A Polyclonal antibody

• Featured Product
• KD/KO Validated

MYO18A Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Publications

17

Reviews (1)

Cat no : 14611-1-AP

Synonyms

CD245, KIAA0216, MAJN, MYO18A, Myosin containing a PDZ domain, myosin XVIIIA, MYSPDZ, SPR210

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Validation Data Gallery

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  WB analysis of Jurkat using 14611-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14611-1-AP (MYO18A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 14611-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14611-1-AP (MYO18A Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 14611-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 14611-1-AP (MYO18A Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 14611-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14611-1-AP (MYO18A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14611-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14611-1-AP (MYO18A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14611-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14611-1-AP (MYO18A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 14611-1-AP

  IP result of anti-MYO18A (IP:14611-1-AP, 3ug; Detection:14611-1-AP 1:300) with HeLa cells lysate 3000ug.

• 

  IHC staining of mouse skeletal muscle using 14611-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14611-1-AP (MYO18A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 14611-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14611-1-AP (MYO18A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 14611-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14611-1-AP (MYO18A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, K-562 cells, Neuro-2a cells, NIH/3T3 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse heart tissue, mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 13 publications below
• IHC: See 1 publications below
• IF: See 5 publications below
• FC: See 1 publications below

Product Information

14611-1-AP targets MYO18A in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MYO18A fusion protein Ag6185
• Full Name: myosin XVIIIA
• Calculated Molecular Weight: 233 kDa
• Observed Molecular Weight: 230 kDa, 190 kDa
• GenBank Accession Number: BC039612
• Gene Symbol: MYO18A
• Gene ID (NCBI): 399687
• RRID: AB_2201447
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYO18A is an unconventional myosin that may play a role in the stabilization and organization of the actin cytoskeleton. Recently MYO18A has been identified as the receptor of SP and is responsible for clearance of SP-A-opsonized bacteria in in vivo infection. Two different isoforms of mouse Myo18A have been identified: Myo18A-alpha and Myo18A-beta as 230- and 190-kDa proteins, respectively. Myo18a-alpha colocalizes with the ER-Golgi complex and with membrane ruffles and filopodia, while Myo18a-beta localizes diffusely to the cytoplasm.

Publications

Species	Application	Title
human	WB	J Clin Invest; A pro-tumorigenic secretory pathway activated by p53 deficiency in lung adenocarcinoma.; Authors - Xiaochao Tan ; KD Validated
human	WB,IHC	Cell Death Differ; The Smad4-MYO18A-PP1A complex regulates β-catenin phosphorylation and pemigatinib resistance by inhibiting PAK1 in cholangiocarcinoma.; Authors - Jialiang Liu ; KD Validated
human	WB,IF	Cell Syst; A Portrait of the Human Organelle Proteome In Space and Time during Cytomegalovirus Infection.; Authors - Pierre M Jean Beltran ; KD Validated
human	WB	Oncoimmunology; Identification of CD245 as myosin 18A, a receptor for surfactant A: A novel pathway for activating human NK lymphocytes.; Authors - A De Masson
human	WB	Diabetes; Unbiased Profiling of the Human Proinsulin Biosynthetic Interaction Network Reveals a Role For Peroxiredoxin 4 in Proinsulin Folding.; Authors - Duc T Tran
rat	WB,IF	J Cell Sci; Pushing myelination: developmental regulation of myosin expression drives oligodendrocyte morphological differentiation; Authors - Helena Sofia Domingues

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sammy (Verified Customer) (04-22-2024)

Detects several bands. One at the correct MW that will need to be validated by KO/KD.

• Applications: Western Blot
• Primary Antibody Dilution: 1 : 1000
• Cell Tissue Type: HUVEC