MYNN Polyclonal antibody

MYNN Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13369-1-AP

Synonyms

MYNN, myoneurin, OSZF, SBBIZ1, ZBTB31

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Validation Data Gallery

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  WB analysis using 13369-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13369-1-AP (MYNN antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 13369-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13369-1-AP (MYNN Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  IHC staining of human placenta using 13369-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13369-1-AP (MYNN antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 13369-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13369-1-AP (MYNN antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: COLO 320 cells, Caco-2 cells, mouse brain tissue
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13369-1-AP targets MYNN in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MYNN fusion protein Ag4188
• Full Name: myoneurin
• Calculated Molecular Weight: 610 aa, 69 kDa
• Observed Molecular Weight: 69 kDa
• GenBank Accession Number: BC033620
• Gene Symbol: MYNN
• Gene ID (NCBI): 55892
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Myoneurin (MYNN) gene locates on 3q26.2 and encodes a member of the BTB/POZ and zinc finger (ZF) domain-containing protein family that is involved in the control of gene expression. MYNN is widely expressed in various tissue types in mammals. MYNN has 4 isoforms with the molecular mass of 10, 57, 65-69 kDa. (PMID: 30120764)