MYL4 Monoclonal antibody, PBS Only

MYL4 Monoclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, pig

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2F5C1

Cat no : 67533-1-PBS

Synonyms

ALC1, AMLC, GT1, MLC1, MYL4, Myosin light chain 4, PRO1957

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Validation Data Gallery

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  WB analysis using 67533-1-Ig (same clone as 67533-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67533-1-Ig (MYL4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67533-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 67533-1-Ig (same clone as 67533-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67533-1-Ig (MYL4 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67533-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 67533-1-Ig (same clone as 67533-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using MYL4 antibody (67533-1-Ig, Clone: 2F5C1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67533-1-PBS in a different storage buffer formulation.

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  FC experiment of C2C12 using 67533-1-Ig (same clone as 67533-1-PBS)

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human MYL4 (67533-1-Ig, Clone:2F5C1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67533-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67533-1-PBS targets MYL4 in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: MYL4 fusion protein Ag19244
• Full Name: myosin, light chain 4, alkali; atrial, embryonic
• Calculated Molecular Weight: 197 aa, 22 kDa
• Observed Molecular Weight: 22-25 kDa
• GenBank Accession Number: BC030228
• Gene Symbol: MYL4
• Gene ID (NCBI): 4635
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

Myosin light‐chain 4 (MYL4) encodes an essential myosin light chain that is expressed in embryonic whole heart and skeletal muscle. It is specifically expressed in human atria after birth. MYL4 is essential for atrial electrical, functional, and structural integrity, and MYL4 dysfunction can produce severe inherited atrial cardiomyopathy.